通过高压液相色谱法及后续电化学检测法测定还原型生物蝶呤。
Determination of reduced biopterins by high pressure liquid chromatography and subsequent electrochemical detection.
作者信息
Bräutigam M, Dreesen R, Herken H
出版信息
Hoppe Seylers Z Physiol Chem. 1982 Mar;363(3):341-3.
PMID:7076129
Abstract
L-erythro-Tetrahydrobiopterin plays an important role as the cofactor of various hydroxylation reactions. We report here the separation of biopterin and its reduced forms by reversed-phase high pressure liquid chromatography. Subsequent electrochemical detection yields a sensitivity at the picomole level for dihydrobiopterin and tetrahydrobiopterin.
摘要
L-赤藓糖型四氢生物蝶呤作为各种羟基化反应的辅助因子发挥着重要作用。我们在此报告通过反相高压液相色谱法分离生物蝶呤及其还原形式。随后的电化学检测对二氢生物蝶呤和四氢生物蝶呤产生皮摩尔级别的灵敏度。
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