Narang H K
Infect Immun. 1982 Apr;36(1):310-9. doi: 10.1128/iai.36.1.310-319.1982.
The morphogenesis of canine distemper virus was studied in Vero cell cultures for 43 days post-inoculation. Active replication of the virus was observed by electron microscopy and assay from 12 h after inoculation on, and peak production was observed on days 5, 14, and 22. From day 28 on, constant but smaller amounts of infectious virus were detected. Two ultrastructural types of intracytoplasmic nucleoprotein filaments were observed; although they first appeared at different times, their subsequent chronological patterns of development were similar. The cells apparently became free of virus by a mechanism of vacuolation. Intranuclear filaments were seen about day 11 and appeared to increase in number thereafter, whereas the infectious titer declined. Possible mechanisms of persistence are discussed in the light of these findings.
在接种后43天内，在Vero细胞培养物中研究了犬瘟热病毒的形态发生。接种后12小时起，通过电子显微镜和检测观察到病毒的活跃复制，在第5天、第14天和第22天观察到病毒产量峰值。从第28天起，检测到恒定但数量较少的感染性病毒。观察到两种超微结构类型的胞质内核蛋白丝；尽管它们最初出现的时间不同，但随后的发育时间模式相似。细胞显然通过空泡化机制清除了病毒。在第11天左右可见核内丝，此后数量似乎增加，而感染滴度下降。根据这些发现讨论了病毒持续存在的可能机制。
