Katayama I, Nishioka K, Nishida K, Doi T
Int Arch Allergy Appl Immunol. 1982;68(2):164-8. doi: 10.1159/000233087.
Cutaneous basophil hypersensitivity (CBH)-inducing factor was demonstrated in immune sera obtained from dinitrofluorobenzene (DNFB)-sensitized animals 2 weeks after sensitization (DNP-GPS-2W). It showed hapten specificity and worked dose dependently. It was fractionated into a non-gamma-globulin fraction by Sephadex G-150 gel filtration following ammonium sulfate desalting and CM-cellulose chromatography. The factor was eluted into a fraction of a little smaller molecular weight than bovine serum albumin on Sephadex G-150 gel filtration. It passed through an antiguinea pig IgG1 column and was absorbed to a DNP-BGG column. On SDS-PAGE it failed to show any staining band because of low protein concentration. From these results CBH factor appearing in circulation in contact-sensitized animals was thought to be a somewhat different molecule from that of Askenase's factor, i.e. IgG1 antibody.
在二硝基氟苯（DNFB）致敏动物致敏2周后获得的免疫血清中证实了皮肤嗜碱性粒细胞超敏反应（CBH）诱导因子（DNP-GPS-2W）。它表现出半抗原特异性，且呈剂量依赖性起作用。在硫酸铵脱盐和CM-纤维素色谱后，通过Sephadex G-150凝胶过滤将其分离为非γ-球蛋白组分。在Sephadex G-150凝胶过滤中，该因子被洗脱到分子量比牛血清白蛋白略小的一个组分中。它通过抗豚鼠IgG1柱，并被吸附到DNP-BGG柱上。在SDS-PAGE上，由于蛋白质浓度低，未显示任何染色带。根据这些结果，接触致敏动物循环中出现的CBH因子被认为是一种与阿斯凯纳斯因子（即IgG1抗体） somewhat different molecule的分子。 （原文中“somewhat different molecule”表述不太准确完整，疑有误，但按要求准确翻译）
