Katayama I, Nishioka K
J Invest Dermatol. 1981 Mar;76(3):197-201. doi: 10.1111/1523-1747.ep12525729.
The guinea pig sera obtained 2 weeks after 1-fluoro-2,4-dinitrobenzene sensitization had ability to suppress elicitation of contact sensitivity when transferred intravenously into contact sensitized guinea pigs. The activity was found only in the sera obtained 2 weeks after the sensitization, whereas the sera obtained 1 and 3 weeks after the sensitization had no effect on contact sensitivity. Pretreatment of cyclophosphamide abolished the generation of the suppressive factor. The suppressive factor was hapten-specific. It was eluted from a Sephadex G-150 column into 7S gamma-globulin fraction and from DEAE ion exchange cellulose column into IgG1 fraction. The suppressive activity was absorbed on a dinitrophenylated bovine gamma globulin Sepharose 4B column and was eluted by either acidic buffered solution or dinitrophenol from the affinity column. It was absorbed by antiguinea pig IgG1-Sepharose and not by antiguinea pig IgG2-Sepharose, Therefore, the suppressive activity found in the sera of 1-fluoro-2, 4-dinitrobenzene sensitized guinea pigs was attributed to IgG1 antihapten antibody.
1-氟-2,4-二硝基苯致敏2周后获得的豚鼠血清，当静脉注射到接触致敏的豚鼠体内时，具有抑制接触敏感性激发的能力。该活性仅在致敏2周后获得的血清中发现，而致敏1周和3周后获得的血清对接触敏感性没有影响。环磷酰胺预处理消除了抑制因子的产生。抑制因子是半抗原特异性的。它从Sephadex G - 150柱洗脱到7Sγ-球蛋白组分中，从DEAE离子交换纤维素柱洗脱到IgG1组分中。抑制活性被二硝基苯基化牛γ球蛋白Sepharose 4B柱吸附，并通过酸性缓冲溶液或二硝基苯酚从亲和柱上洗脱下来。它被抗豚鼠IgG1 - Sepharose吸附，而不被抗豚鼠IgG2 - Sepharose吸附，因此，在1-氟-2,4-二硝基苯致敏豚鼠血清中发现的抑制活性归因于IgG1抗半抗原抗体。
