Kolega J, Shure M S, Chen W T, Young N D
J Cell Sci. 1982 Apr;54:23-34. doi: 10.1242/jcs.54.1.23.
Interference-reflection microscopy combined with time-lapse cinemicrography was used to examine the relationship between cell-to-substratum contact patterns and the speeds of translocation for a variety of cell types. Rapid translocation of amphibian leukocytes (average speed = 9.0 micron/min), amphibian epidermal cells (7 micron/min) and teleost epidermal cells (7 micron/min) was found to correlate with patterns of broad grey close contacts. Similar contact patterns were found under freshly seeded (2 h) chick heart fibroblasts (moving 1-3 micron/min), the rapidly advancing (1-5 micron/min) margin of spreading human WI-38 fibroblasts, and isolated MDCK canine epithelial cells (0.5-1.0 micron/min). Conversely, numerous dark streaks of focal contact were found associated with the slow rate of translocation displayed by older cultures (72 h) of chick fibroblasts (less than 0.1 micron/min), well-spread WI-38 cells (less than or equal to 0.3 micron/min) and confluent MDCK cells (less than 0.01 micron/min). It is concluded that close contacts, but not focal contacts, are associated with rapid cellular translocation, and that the build-up of focal contacts is associated with reduced cellular translocation and maintenance of the spread cell shape.
采用干涉反射显微镜结合延时显微电影技术，研究了多种细胞类型中细胞与基质接触模式与转运速度之间的关系。发现两栖类白细胞（平均速度 = 9.0微米/分钟）、两栖类表皮细胞（7微米/分钟）和硬骨鱼表皮细胞（7微米/分钟）的快速转运与广泛的灰色紧密接触模式相关。在新鲜接种（2小时）的鸡心脏成纤维细胞（移动速度为1 - 3微米/分钟）、正在快速扩展（1 - 5微米/分钟）的人WI - 38成纤维细胞边缘以及分离的MDCK犬上皮细胞（0.5 - 1.0微米/分钟）中也发现了类似的接触模式。相反，在鸡成纤维细胞的老龄培养物（72小时）（速度小于0.1微米/分钟）、充分铺展的WI - 38细胞（小于或等于0.3微米/分钟）和汇合的MDCK细胞（小于0.01微米/分钟）中，发现大量局灶接触的暗条纹与缓慢的转运速率相关。得出的结论是，紧密接触而非局灶接触与细胞的快速转运相关，并且局灶接触的积累与细胞转运减少以及铺展细胞形状的维持相关。
