14C-交替单甲醚的分布、消除与代谢

The distribution, elimination, and metabolism of 14C-alternariol monomethyl ether.

作者信息

Pollock G A, DiSabatino C E, Heimsch R C, Coulombe R A

出版信息

J Environ Sci Health B. 1982;17(2):109-24. doi: 10.1080/03601238209372306.

DOI:10.1080/03601238209372306

Abstract

Adult male Sprague-Dawley rats were orally administered 14C-alternariol monomethyl ether (AME) in olive oil and housed in glass metabolism cages. Urine, feces, and expired CO2 were monitored for the presence of radioactivity. The majority of activity was excreted in the feces apparently unabsorbed. Urine excretion accounted for less than 10% of the administered dose. A substantial amount of "absorbed" activity was excreted in expired CO2. TLC autoradiography of urine extracts revealed extensive metabolism, but little metabolism was evident in feces extracts. Incubation of AME with rat liver post-mitochondrial supernatant also resulted in substantial metabolism.

摘要

成年雄性斯普拉格-道利大鼠经口给予溶于橄榄油的14C-单甲基交替单端孢霉烯（AME），并饲养在玻璃代谢笼中。监测尿液、粪便和呼出的二氧化碳中是否存在放射性。大部分放射性显然未被吸收，而是随粪便排出。尿液排泄量占给药剂量的不到10%。大量“吸收”的放射性通过呼出的二氧化碳排出。尿液提取物的薄层层析放射自显影显示有广泛的代谢，但粪便提取物中几乎没有明显的代谢。AME与大鼠肝脏线粒体后上清液孵育也导致大量代谢。