Maier C E, Singer M
J Exp Zool. 1982 Jan 10;219(1):111-4. doi: 10.1002/jez.1402190114.
Newts with bilaterally amputated forelimbs were exposed to either continuous light (LL), total darkness (DD), or continuous light with the dorsal head epithelium painted with an India ink and Nile blue sulfate mixture (LL-II-NBS) that limited light penetration through the skull to the pineal. The LL-II-NBS animals regenerated their forelimbs more slowly then their counterparts in LL.
双侧前肢被截断的蝾螈被置于持续光照(LL)、完全黑暗(DD)或持续光照环境中,同时用印度墨水和硫酸尼罗蓝混合物涂抹其头部背侧上皮(LL-II-NBS),这种混合物限制了光线透过头骨到达松果体。与处于持续光照环境中的蝾螈相比,处于LL-II-NBS环境中的蝾螈前肢再生速度更慢。