Toop J, Burke R E, Dum R P, O'Donovan M J, Smith C B
J Neurosci Methods. 1982 Mar;5(3):283-9. doi: 10.1016/0165-0270(82)90080-2.
2-Deoxy-D-[l-14C]glucose (2DG) was given intravenously during repetitive stimulation of single motor units in adult cats and autoradiographs were made of frozen sections of the target muscles in order to evaluate methods designed to improve the spatial resolution of [14C]2DG autoradiography. With the modifications used, acutely active muscle fibers, independently identified by depletion of intrafiber glycogen, were associated with highly localized accumulations of silver grains over the depleted fibers. The results indicate that [14C]2DG autoradiography can successfully identify individual active muscle fibers and might in principle be used to obtain quantitative data about rates of glucose metabolism in single muscle fibers of defined histochemical type. The modifications may be applicable also to other tissues to give improved spatial resolution with [14C]-labeled metabolic markers.
在成年猫的单个运动单位重复刺激期间静脉注射2-脱氧-D-[l-14C]葡萄糖（2DG），并对目标肌肉的冰冻切片制作放射自显影片，以评估旨在提高[14C]2DG放射自显影空间分辨率的方法。采用这些改进方法后，通过纤维内糖原耗竭独立鉴定出的急性活跃肌纤维，与耗竭纤维上银颗粒的高度局部积累相关。结果表明，[14C]2DG放射自显影可以成功识别单个活跃肌纤维，原则上可用于获取有关特定组织化学类型单根肌纤维葡萄糖代谢率的定量数据。这些改进方法也可能适用于其他组织，以提高[14C]标记代谢标记物的空间分辨率。
