Tulp O L, Gregory M H, Danforth E
Life Sci. 1982 May 3;30(18):1525-30. doi: 10.1016/0024-3205(82)90239-9.
The characteristics of regional brown (BAT) and white adipose tissue (WAT) growth and of thermogenesis following experimental overfeeding were studied in groups of male Sprague-Dawley rats fed lab chow or cafeteria diets for 8 weeks postweaning. Regional BAT and WAT growth was determined by dissection and weighing, and thermogenesis was characterized by measurements of resting and norepinephrine (NE)-stimulated oxygen consumption, of serum thyroid hormone concentrations, and of 24-hour urinary NE excretion levels. Cafeteria feeding resulted in a 113% increase in total BAT, with the most prominent increases in the interscapular, thoracic, and perirenal regions. Retroperitoneal, epididymal, and omental WAT were significantly greater in cafeteria than in chow-fed rats. Resting oxygen consumption of cafeteria-fed rads increased by 10% and NE excretion by 64% compared to chow-fed controls, while serum T3 concentrations were nearly doubled in the cafeteria-fed rats. The thermogenic response to NE injection in cafeteria-fed rats was 102% of their resting levels, compared to a 51% increase in the chow-fed controls. The results indicate that increased BAT growth occurs in all primary BAT depots following cafeteria-feeding in rats, and that the greater BAT mass is qualitatively proportional to their greater capacity for non-shivering thermogenesis. Also, the increased NE excretion and greater serum T3 concentration are consistent with increased sympathetic and thyroidal activity and may in part explain the thermogenic response to diet in the rat.
对断奶后以实验室饲料或自助餐饮食喂养8周的雄性斯普拉格-道利大鼠进行分组研究，观察实验性过度喂养后棕色脂肪组织（BAT）和白色脂肪组织（WAT）的局部生长特征以及产热情况。通过解剖和称重确定BAT和WAT的局部生长情况，通过测量静息和去甲肾上腺素（NE）刺激后的耗氧量、血清甲状腺激素浓度以及24小时尿NE排泄水平来表征产热情况。自助餐喂养使BAT总量增加了113%，其中肩胛间、胸部和肾周区域的增加最为显著。与喂食普通饲料的大鼠相比，自助餐喂养大鼠的腹膜后、附睾和网膜WAT明显更多。与喂食普通饲料的对照组相比，自助餐喂养大鼠的静息耗氧量增加了10%，NE排泄增加了64%，而自助餐喂养大鼠的血清T3浓度几乎增加了一倍。与喂食普通饲料的对照组增加51%相比，自助餐喂养大鼠对NE注射的产热反应是其静息水平的102%。结果表明，大鼠自助餐喂养后所有主要BAT储存部位的BAT生长均增加，且更大的BAT量在质量上与其更高的非颤抖产热能力成正比。此外，NE排泄增加和血清T3浓度升高与交感神经和甲状腺活动增加一致，可能部分解释了大鼠对饮食的产热反应。
