Marchington D, Rothwell N J, Stock M J, York D A
Am J Physiol. 1986 Apr;250(4 Pt 1):E362-6. doi: 10.1152/ajpendo.1986.250.4.E362.
Resting oxygen consumption was elevated by 30% in young rats fed a cafeteria diet compared with their chow-fed controls and by 22% in cafeteria-fed, adrenalectomized (ADX) rats compared with the ADX chow-fed group, but injection of propranolol reduced oxygen consumption in the cafeteria-fed animals and abolished these differences. Brown adipose tissue (BAT) mass was increased by cafeteria feeding, and the activity of the mitochondrial proton conductance pathway (assessed from purine nucleotide binding) was enhanced by adrenalectomy and by cafeteria feeding. Norepinephrine turnover in BAT (determined from the time-dependent loss of tissue [3H]norepinephrine specific activity) was increased by 105% in sham-operated, cafeteria-fed rats, by 142% in chow-fed ADX rats, and by 400% in cafeteria-fed ADX rats, compared with chow-fed controls. Cardiac norepinephrine turnover was elevated by 80% in sham-operated, cafeteria-fed rats, but unaffected by adrenalectomy. These data indicate that the enhanced thermogenesis and BAT activity induced by adrenalectomy in chow- or cafeteria-fed rats is due to increased sympathetic activity in the tissue.
与喂食普通饲料的对照幼鼠相比，喂食自助式饮食的幼鼠静息氧耗量升高了30%；与喂食普通饲料的肾上腺切除（ADX）大鼠相比，喂食自助式饮食的ADX大鼠静息氧耗量升高了22%，但注射普萘洛尔可降低喂食自助式饮食动物的氧耗量，并消除这些差异。自助式饮食可增加棕色脂肪组织（BAT）的质量，肾上腺切除术和自助式饮食均可增强线粒体质子传导途径的活性（通过嘌呤核苷酸结合评估）。与喂食普通饲料的对照大鼠相比，假手术、喂食自助式饮食的大鼠BAT中的去甲肾上腺素周转率（根据组织[3H]去甲肾上腺素比活性随时间的损失确定）增加了105%，喂食普通饲料的ADX大鼠增加了142%，喂食自助式饮食的ADX大鼠增加了400%。假手术、喂食自助式饮食的大鼠心脏去甲肾上腺素周转率升高了80%，但不受肾上腺切除术的影响。这些数据表明，肾上腺切除术在喂食普通饲料或自助式饮食的大鼠中诱导的产热增加和BAT活性增强是由于组织中交感神经活性增加所致。
