Rietveld K, Van Poelgeest R, Pleij C W, Van Boom J H, Bosch L
Nucleic Acids Res. 1982 Mar 25;10(6):1929-46. doi: 10.1093/nar/10.6.1929.
The 3' terminus of TYMV RNA, which possesses tRNA-like properties, has been studied. A 3' terminal fragment of 112 nucleotides was obtained by cleavage with RNase H after hybridization of a synthetic oligodeoxynucleotide to the viral RNA. The accessibility of cytidine and adenosine residues was probed with chemical modification. Enzymatic digestion studies were performed with RNase T1, nuclease S1 and the double-strand specific RNase from the venom of the cobra Naja naja oxiana. A model is proposed for the secondary structure of the 3' terminal region of TYMV RNA comprising 86 nucleotides. The main feature of this secondary structure is the absence of a conventional acceptor stem as present in canonical tRNA. However, the terminal 42 nucleotides can be folded in a tertiary structure which bears strong resemblance with the acceptor arm of canonical tRNA. Comparison of this region of TYMV RNA with that of other RNAs from both the tymovirus group and the tobamovirus group gives support to our proposal for such a three-dimensional arrangement. The consequences for the recognition by TYMV RNA of tRNA-specific enzymes is discussed.
对具有类似tRNA特性的芜菁黄花叶病毒（TYMV）RNA的3'末端进行了研究。通过将合成的寡脱氧核苷酸与病毒RNA杂交后用RNase H切割，获得了一个112个核苷酸的3'末端片段。用化学修饰法探测胞嘧啶和腺苷残基的可及性。用RNase T1、核酸酶S1和眼镜蛇纳吉亚纳亚奥克西纳毒液中的双链特异性RNase进行了酶切研究。提出了一个包含86个核苷酸的TYMV RNA 3'末端区域二级结构模型。这种二级结构的主要特征是不存在典型tRNA中存在的传统受体茎。然而，末端的42个核苷酸可以折叠成与典型tRNA的受体臂非常相似的三级结构。将TYMV RNA的这一区域与芜菁黄花叶病毒组和烟草花叶病毒组的其他RNA的该区域进行比较，支持了我们对这种三维排列的提议。讨论了TYMV RNA对tRNA特异性酶识别的影响。
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