Joshi S, Chapeville F, Haenni A L
Nucleic Acids Res. 1982 Mar 25;10(6):1947-62. doi: 10.1093/nar/10.6.1947.
This paper describes the minimum length of the turnip yellow mosaic virus (TYMV) RNA necessary to fulfill the tRNA-like properties of the viral RNA: 50 to 75 nucleotides and 86 nucleotides from the 3' end of TYMV RNA are sufficient for adenylation and valylation respectively by the Escherichia coli system. The size of the tRNA-like fragments obtained in vitro in the presence of an E. coli, a reticulocyte or a chinese cabbage leaf extract has also been determined. Among the major fragments liberated from the 3' end of TYMV RNA by the three systems are fragments of 117 and 112 nucleotides. In addition, the E. coli extract liberates fragments of 139 and 61 nucleotides, and the reticulocyte lysate fragments of 109, 94, 84, 73 and 46 nucleotides. The cleavage of the viral RNA by several systems in vitro to yield RNA fragments encompassing the tRNA-like sequence suggests that such fragments might also be liberated in vivo.
本文描述了芜菁黄花叶病毒（TYMV）RNA为实现病毒RNA的tRNA样特性所必需的最短长度：来自TYMV RNA 3'端的50至75个核苷酸和86个核苷酸分别足以被大肠杆菌系统进行腺苷酸化和缬氨酸化。还测定了在大肠杆菌、网织红细胞或大白菜叶提取物存在下体外获得的tRNA样片段的大小。在这三种系统从TYMV RNA 3'端释放的主要片段中，有117和112个核苷酸的片段。此外，大肠杆菌提取物释放出139和61个核苷酸的片段，网织红细胞裂解物释放出109、94、84、73和46个核苷酸的片段。几种系统在体外对病毒RNA进行切割以产生包含tRNA样序列的RNA片段，这表明此类片段在体内也可能被释放出来。
