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来自纤细短膜虫的超长5.8S核糖体RNA的核苷酸序列。

Nucleotide sequence of an exceptionally long 5.8S ribosomal RNA from Crithidia fasciculata.

作者信息

Schnare M N, Gray M W

出版信息

Nucleic Acids Res. 1982 Mar 25;10(6):2085-92. doi: 10.1093/nar/10.6.2085.

DOI:10.1093/nar/10.6.2085
PMID:7079176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320590/
Abstract

In Crithidia fasciculata, a trypanosomatid protozoan, the large ribosomal subunit contains five small RNA species (e, f, g, i, j) in addition to 5S rRNA [Gray, M.W. (1981) Mol. Cell. Biol. 1, 347-357]. The complete primary sequence of species i is shown here to be pAACGUGUmCGCGAUGGAUGACUUGGCUUCCUAUCUCGUUGA ... AGAmACGCAGUAAAGUGCGAUAAGUGGUApsiCAAUUGmCAGAAUCAUUCAAUUACCGAAUCUUUGAACGAAACGG ... CGCAUGGGAGAAGCUCUUUUGAGUCAUCCCCGUGCAUGCCAUAUUCUCCAmGUGUCGAA(C)OH. This sequence establishes that species i is a 5.8S rRNA, despite its exceptional length (171-172 nucleotides). The extra nucleotides in C. fasciculata 5.8S rRNA are located in a region whose primary sequence and length are highly variable among 5.8S rRNAs, but which is capable of forming a stable hairpin loop structure (the "G+C-rich hairpin"). The sequence of C. fasciculata 5.8S rRNA is no more closely related to that of another protozoan, Acanthamoeba castellanii, than it is to representative 5.8S rRNA sequences from the other eukaryotic kingdoms, emphasizing the deep phylogenetic divisions that seem to exist within the Kingdom Protista.

摘要

在克氏锥虫（一种锥虫类原生动物）中，大核糖体亚基除了含有5S rRNA外，还包含五种小RNA种类（e、f、g、i、j）[格雷，M.W.（1981年）《分子细胞生物学》1，347 - 357]。这里展示了种类i的完整一级序列为pAACGUGUmCGCGAUGGAUGACUUGGCUUCCUAUCUCGUUGA...AGAmACGCAGUAAAGUGCGAUAAGUGGUApsiCAAUUGmCAGAAUCAUUCAAUUACCGAAUCUUUGAACGAAACGG...CGCAUGGGAGAAGCUCUUUUGAGUCAUCCCCGUGCAUGCCAUAUUCUCCAmGUGUCGAA(C)OH。该序列确定种类i是一种5.8S rRNA，尽管其长度异常（171 - 172个核苷酸）。克氏锥虫5.8S rRNA中的额外核苷酸位于一个区域，该区域的一级序列和长度在5.8S rRNAs中高度可变，但能够形成稳定的发夹环结构（“富含G + C的发夹”）。克氏锥虫5.8S rRNA的序列与另一种原生动物卡氏棘阿米巴的序列相比，与其他真核生物界代表性5.8S rRNA序列的关系并不更密切，这强调了原生生物界内似乎存在的深刻系统发育分歧。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660d/320590/457d95eba068/nar00375-0260-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660d/320590/940adb6aafe8/nar00375-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660d/320590/457d95eba068/nar00375-0260-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660d/320590/940adb6aafe8/nar00375-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660d/320590/457d95eba068/nar00375-0260-b.jpg

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2
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