Mackay R M, Spencer D F, Doolittle W F, Gray M W
Eur J Biochem. 1980 Dec;112(3):561-76. doi: 10.1111/j.1432-1033.1980.tb06122.x.
The nucleotide sequences of wheat embryo 5.8-S and 5-S rRNAs have been determined with the use of several techniques, including classic analysis of oligonucleotides generated by ribonuclease T1 and resolution on gels of terminally labelled RNA partially degraded with ribonucleases or with chemical reagents. The sequence of wheat embryo 5.8-S rRNA was found to be (formula: see text). This sequence is compared to 5-S rRNA sequences previously published for wheat and several other angiosperms.
利用多种技术测定了小麦胚5.8 - S和5 - S核糖体RNA的核苷酸序列,这些技术包括对核糖核酸酶T1产生的寡核苷酸进行经典分析,以及对用核糖核酸酶或化学试剂部分降解的末端标记RNA在凝胶上进行分离。发现小麦胚5.8 - S核糖体RNA的序列为(分子式:见正文)。将该序列与先前发表的小麦及其他几种被子植物的5 - S核糖体RNA序列进行了比较。