空肠回肠旁路术后胃泌素、胰高血糖素和血管活性肠肽的循环免疫反应性。

Circulating immunoreactivities of gastrin, glucagon and VIP after jejuno-ileal bypass.

作者信息

Kulneff-Herlin A, Herlin P, Chen M H, Gimmon Z, Murphy R F, Joffe S N

出版信息

Peptides. 1982 Jan-Feb;3(1):91-3. doi: 10.1016/0196-9781(82)90148-6.

Abstract

Seven Sprague-Dawley rats (404-440 g) underwent a 90% jejuno-ileal bypass (JIB); the functional loop consisted of 1/3 ileum and 2/3 jejunum with the bypassed loop being anastomosed to the ascending colon. Seven control rats were sham-operated. After 35 days, the rats were fasted 18 hours and venous blood was collected. Immunoreactivity of gastrin, measured with an antibody binding equally to G17 and G34, was higher in the plasma of the JIB (256 +/- 55 SEM pg/ml) than control (85 +/- 9 pg/ml) rats. This agrees with recent human studies but is in conflict with results in less mature rats. VIP levels were not significantly different. Glucagon-like immunoreactivity measured with antibodies specific for the C- and N-terminal regions of the hormone, respectively, were also higher in the JIB (510 +/- 40 and 129 +/- 15 pg/ml) rats.

摘要

七只斯普拉格-道利大鼠（体重404 - 440克）接受了90%的空肠-回肠旁路手术（JIB）；功能性肠袢由1/3的回肠和2/3的空肠组成，被旁路的肠袢与升结肠吻合。七只对照大鼠接受假手术。35天后，大鼠禁食18小时并采集静脉血。用与G17和G34具有同等结合力的抗体测量的胃泌素免疫反应性，在接受JIB手术的大鼠血浆中（256±55 SEM pg/ml）高于对照大鼠（85±9 pg/ml）。这与最近的人体研究结果一致，但与幼龄大鼠的结果相矛盾。血管活性肠肽（VIP）水平无显著差异。分别用针对该激素C端和N端区域的特异性抗体测量的胰高血糖素样免疫反应性，在接受JIB手术的大鼠中（510±40和129±15 pg/ml）也较高。