Tranberg K G, Tabata K, Chen M H, Murphy R F, Fischer J E, Joffe S N
Ann Surg. 1985 Mar;201(3):300-4. doi: 10.1097/00000658-198503000-00007.
In the rat, about 80% of the intestinal content of secretin immunoreactivity is found distal to the duodenum with a peak in the ileum. This work therefore studied whether the secretin immunoreactivity in distal ileum could be released by substances normally found in the intestinal lumen. An isolated loop of ileum or duodenum was perfused with 100 mM HCl, 3 mM HCl, 50 mM taurocholate, 50 mM oleate, 308 mM glucose, 273 mM amino acids, or 308 mM NaCl in anesthetized rats. Release of secretin immunoreactivity was estimated by the integrated response above basal in portal blood. Substantial release of secretin immunoreactivity was seen after both ileal and duodenal perfusion with concentrated HCl and taurocholate, whereas perfusion with oleate was followed by a lesser response. The response to taurocholate was slower than that to HCl, but more likely represents a physiologic mechanism. The results show that the distal small intestine is capable of contributing to secretin immunoreactivity in the circulation and it is speculated that bile salts and secretin constitute parts of a negative feedback loop.
在大鼠中，约80%的促胰液素免疫反应性肠内容物位于十二指肠远端，在回肠达到峰值。因此，这项研究探讨了回肠远端的促胰液素免疫反应性是否能被肠腔内常见的物质释放出来。在麻醉的大鼠中，用100 mM盐酸、3 mM盐酸、50 mM牛磺胆酸盐、50 mM油酸盐、308 mM葡萄糖、273 mM氨基酸或308 mM氯化钠灌注分离的回肠或十二指肠环。通过门静脉血中高于基础值的综合反应来估计促胰液素免疫反应性的释放。在用浓盐酸和牛磺胆酸盐灌注回肠和十二指肠后，均观察到促胰液素免疫反应性的大量释放，而用油酸盐灌注后反应较小。对牛磺胆酸盐的反应比对盐酸的反应慢，但更可能代表一种生理机制。结果表明，远端小肠能够对循环中的促胰液素免疫反应性产生影响，推测胆盐和促胰液素构成负反馈回路的一部分。
