红细胞溶血动力学在复杂心脏毒素混合物纯化中的应用。

Use of erythrocyte hemolysis kinetics in the purification of complex cardiotoxin mixtures.

作者信息

Zusman N, Cafmeyer N, Hudson R A

出版信息

Toxicon. 1982;20(2):517-20. doi: 10.1016/0041-0101(82)90018-6.

Abstract

A human erythrocyte hemolysis kinetic method provides a useful way to follow the purification of cobra venom cardiotoxins or other hemolytic factors. Initial rates of hemolysis, measured as hemoglobin released with time for the separated cardiotoxins from the venom of the Thailand cobra Naja naja siamensis, vary over a greater range than do other commonly used measures of their biological activity. Recovery of the hemolytic activity of the gross cardiotoxin fraction in the subsequently separated fractions has been demonstrated. The method employs submilligram quantities of mixed or purified cardiotoxins.

摘要

一种人体红细胞溶血动力学方法为跟踪眼镜蛇毒心脏毒素或其他溶血因子的纯化过程提供了一种有用的途径。以泰国眼镜蛇（Naja naja siamensis）毒液中分离出的心脏毒素随时间释放的血红蛋白量来衡量的初始溶血速率，其变化范围比其他常用的生物活性测量方法更大。已证明在随后分离的组分中总心脏毒素组分的溶血活性得以恢复。该方法使用亚毫克量的混合或纯化心脏毒素。