与福氏耐格里阿米巴表面膜相关的一种热稳定细胞溶解蛋白的激活。
Activation of a heat-stable cytolytic protein associated with the surface membrane of Naegleria fowleri.
作者信息
Lowrey D M, McLaughlin J
出版信息
Infect Immun. 1985 Nov;50(2):478-82. doi: 10.1128/iai.50.2.478-482.1985.
Abstract
Surface membrane-enriched fractions of Naegleria fowleri obtained after isopycnic centrifugation experiments contain a potent cytolytic activity as determined by hemolysis and 51Cr release assays. This surface membrane cytolysin was unaffected by a treatment at 75 degrees C for 30 min and accounted for 70 to 90% of cytolysis by whole-cell lysates of amoebae. This heat resistance as well as intimate membrane association distinguished the surface membrane cytolytic activity from a second heat-labile cytolytic activity which appears to be latent within lysosomes. The surface membrane cytolysin was found to be specifically activated by diluted samples of lysosomal fractions. The possible role of this surface membrane cytotoxin in the pathogenicity of N. fowleri is discussed.
摘要
通过等密度离心实验获得的福氏耐格里阿米巴表面膜富集组分，经溶血和⁵¹Cr释放试验测定，具有强大的细胞溶解活性。这种表面膜溶细胞素在75℃处理30分钟后不受影响，并且占变形虫全细胞裂解物细胞溶解的70%至90%。这种耐热性以及与膜的紧密结合，使表面膜细胞溶解活性与第二种对热不稳定的细胞溶解活性区分开来，后者似乎潜伏在溶酶体内。发现表面膜溶细胞素可被溶酶体组分的稀释样品特异性激活。讨论了这种表面膜细胞毒素在福氏耐格里阿米巴致病性中的可能作用。
相似文献
1
Activation of a heat-stable cytolytic protein associated with the surface membrane of Naegleria fowleri.与福氏耐格里阿米巴表面膜相关的一种热稳定细胞溶解蛋白的激活。
Infect Immun. 1985 Nov;50(2):478-82. doi: 10.1128/iai.50.2.478-482.1985.
2
Soluble amoebicidal factors mediate cytolysis of Naegleria fowleri by activated macrophages.
Cell Immunol. 1986 Aug;101(1):62-71. doi: 10.1016/0008-8749(86)90186-3.
3
A multicomponent hemolytic system in the pathogenic amoeba Naegleria fowleri.致病性变形虫福氏耐格里阿米巴中存在的多组分溶血系统。
Infect Immun. 1984 Sep;45(3):731-6. doi: 10.1128/iai.45.3.731-736.1984.
4
5
Cytopathogenicity of Naegleria fowleri for cultured rat neuroblastoma cells.
J Protozool. 1985 Feb;32(1):176-80. doi: 10.1111/j.1550-7408.1985.tb03034.x.
6
Cytolytic activity of Naegleria fowleri cell-free extract.
J Protozool. 1986 Nov;33(4):498-502. doi: 10.1111/j.1550-7408.1986.tb05649.x.
7
Stability, antigenicity, and aggregation of Moraxella bovis cytolysin after purification and storage.
Am J Vet Res. 2004 Jul;65(7):977-83. doi: 10.2460/ajvr.2004.65.977.
8
Membrane vesiculation of Naegleria fowleri amoebae as a mechanism for resisting complement damage.福氏耐格里阿米巴的膜囊泡化作为抵抗补体损伤的一种机制。
J Immunol. 1994 Mar 15;152(6):2952-9.
9
The pathogenic amoeboflagellate Naegleria fowleri: environmental isolations, competitors, ecologic interactions, and the flagellate-empty habitat hypothesis.致病性变形鞭毛虫福氏耐格里阿米巴：环境分离、竞争者、生态相互作用及鞭毛虫空生境假说
J Protozool. 1983 May;30(2):403-9. doi: 10.1111/j.1550-7408.1983.tb02939.x.
10
Observations by immunofluorescence microscopy and electron microscopy on the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures.
J Med Microbiol. 1979 Aug;12(3):363-71. doi: 10.1099/00222615-12-3-363.
引用本文的文献
1
A Novel Cysteine Protease Inhibitor of That Is Specifically Expressed during Encystation and at Mature Cysts.一种新型的半胱氨酸蛋白酶抑制剂，其在包囊化过程中及成熟包囊中特异性表达。
Pathogens. 2021 Mar 24;10(4):388. doi: 10.3390/pathogens10040388.
2
Genome-wide identification of pathogenicity factors of the free-living amoeba Naegleria fowleri.自由生活变形虫福氏耐格里阿米巴致病因素的全基因组鉴定。
BMC Genomics. 2014 Jun 19;15(1):496. doi: 10.1186/1471-2164-15-496.
3
Cytopathic changes in rat microglial cells induced by pathogenic Acanthamoeba culbertsoni: morphology and cytokine release.致病性卡氏棘阿米巴诱导的大鼠小胶质细胞病变：形态学与细胞因子释放
Clin Diagn Lab Immunol. 2001 Jul;8(4):837-40. doi: 10.1128/CDLI.8.4.837-840.2001.
4
Apoptosis of primary-culture rat microglial cells induced by pathogenic Acanthamoeba spp.致病性棘阿米巴原虫诱导的原代培养大鼠小胶质细胞凋亡
Clin Diagn Lab Immunol. 2000 May;7(3):510-4. doi: 10.1128/CDLI.7.3.510-514.2000.
5
Biology of Naegleria spp.耐格里属生物
Microbiol Rev. 1988 Mar;52(1):114-33. doi: 10.1128/mr.52.1.114-133.1988.
6
Cloning and characterization of transcripts showing virulence-related gene expression in Naegleria fowleri.福氏耐格里阿米巴中显示毒力相关基因表达的转录本的克隆与表征
Infect Immun. 1992 Jun;60(6):2418-24. doi: 10.1128/iai.60.6.2418-2424.1992.
本文引用的文献
1
CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA.从一名急性白血病患儿外周血中连续培养人淋巴细胞
Cancer. 1965 Apr;18:522-9. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j.
2
Degradation of human myelin phospholipids by phospholipase-enriched culture media of pathogenic Naegleria fowleri.致病性福氏耐格里阿米巴富含磷脂酶的培养基对人髓磷脂磷脂的降解作用
Biochim Biophys Acta. 1982 Sep 14;712(3):698-701. doi: 10.1016/0005-2760(82)90300-9.
3
Use of erythrocyte hemolysis kinetics in the purification of complex cardiotoxin mixtures.红细胞溶血动力学在复杂心脏毒素混合物纯化中的应用。
Toxicon. 1982;20(2):517-20. doi: 10.1016/0041-0101(82)90018-6.
4
Cytopathogenicity of Naegleria fowleri and Naegleria gruberi for established mammalian cell cultures.福氏耐格里阿米巴和格氏耐格里阿米巴对已建立的哺乳动物细胞培养物的细胞致病性。
J Parasitol. 1982 Dec;68(6):1110-6.
5
Primary amebic meningoencephalitis and the biology of Naegleria fowleri.原发性阿米巴脑膜脑炎与福氏耐格里阿米巴的生物学
Annu Rev Microbiol. 1982;36:101-23. doi: 10.1146/annurev.mi.36.100182.000533.
6
Cytolytic activity of purified cytoplasmic granules from cytotoxic rat large granular lymphocyte tumors.来自细胞毒性大鼠大颗粒淋巴细胞肿瘤的纯化细胞质颗粒的细胞溶解活性。
J Exp Med. 1984 Jul 1;160(1):75-93. doi: 10.1084/jem.160.1.75.
7
Sucker-like structures on the pathogenic amoeba Naegleria fowleri.致病性阿米巴福氏耐格里变形虫上的吸盘状结构。
Appl Environ Microbiol. 1984 Jan;47(1):12-4. doi: 10.1128/aem.47.1.12-14.1984.
8
A simple and reliable method for cloning Naegleria fowleri.一种简单可靠的福氏耐格里阿米巴克隆方法。
J Parasitol. 1984 Dec;70(6):991-2.
9
A multicomponent hemolytic system in the pathogenic amoeba Naegleria fowleri.致病性变形虫福氏耐格里阿米巴中存在的多组分溶血系统。
Infect Immun. 1984 Sep;45(3):731-6. doi: 10.1128/iai.45.3.731-736.1984.
10
A glycosylated protoxin in killer yeast: models for its structure and maturation.杀伤酵母中的一种糖基化原毒素：其结构与成熟的模型
Cell. 1983 Jan;32(1):169-80. doi: 10.1016/0092-8674(83)90507-x.
Zotero 插件