Weil G J, Powers K G, Parbuoni E L, Line B R, Furrow R D, Ottesen E A
Am J Trop Med Hyg. 1982 May;31(3 Pt 1):477-85.
Antimicrofilarial immunity was studied in Dirofilaria immitis-infected dogs in order to better understand amicrofilaremic filariasis in man. Sera from dogs with amicrofilaremic infections contained IgG antibodies specific for microfilarial surface antigens detectable by immunofluorescence and in vitro leukocyte adherence. In vivo immune mechanisms were studied by injecting 51Cr-labeled microfilariae (MF) into infected and uninfected dogs. Injected MF were concentrated in lung, liver, spleen, and kidneys of normal and microfilaremic dogs, but circulated throughout the 5-hour study period. In contrast, injected MF were rapidly cleared (15-30 min) from the blood of amicrofilaremic-infected dogs. Tissue radioactivity and histopathology indicated that injected MF were trapped and destroyed in the lungs of these dogs. Antibody-dependent clearance and destruction of MF is a potent anti-helminth effector mechanism in canine dirofilariasis. Similar events are likely to occur in amicrofilameric filariasis in humans.
为了更好地理解人类的无微丝蚴血症丝虫病，对感染犬恶丝虫的狗的抗微丝蚴免疫进行了研究。来自无微丝蚴血症感染犬的血清含有针对微丝蚴表面抗原的IgG抗体，可通过免疫荧光和体外白细胞黏附检测到。通过将51Cr标记的微丝蚴（MF）注射到感染和未感染的狗体内来研究体内免疫机制。注射的MF集中在正常和无微丝蚴血症犬的肺、肝、脾和肾脏中，但在整个5小时的研究期间都在循环。相比之下，注射的MF在无微丝蚴血症感染犬的血液中迅速清除（15 - 30分钟）。组织放射性和组织病理学表明，注射的MF在这些犬的肺部被捕获并破坏。抗体依赖性清除和破坏MF是犬恶丝虫病中一种有效的抗蠕虫效应机制。类似的事件可能发生在人类的无微丝蚴血症丝虫病中。
