烟草细胞和番茄子房鸟氨酸脱羧酶的特性研究
Characterization of ornithine decarboxylase of tobacco cells and tomato ovaries.
作者信息
Heimer Y M, Mizrahi Y
出版信息
Biochem J. 1982 Feb 1;201(2):373-6. doi: 10.1042/bj2010373.
Abstract
Some characteristics of L-ornithine decarboxylase of tomato ovaries and tobacco cells are described. The enzyme has a pH optimum of 8.0. It requires pyridoxal phosphate and thiol reagent (dithiothreitol) for activity. It is specific for L-ornithine and has an apparent Km of 1.4 X 10-4 M. It has an apparent molecular weight of 107000. Putrescine inhibited the activity in vitro. Spermidine and spermine also inhibit the enzyme, but less effectively. It is concluded that the enzyme is similar to that of mammalian origin and likewise fulfils a function related to cell proliferation.
摘要
描述了番茄子房和烟草细胞中L-鸟氨酸脱羧酶的一些特性。该酶的最适pH为8.0。其活性需要磷酸吡哆醛和硫醇试剂（二硫苏糖醇）。它对L-鸟氨酸具有特异性，表观Km为1.4×10-4M。其表观分子量为107000。腐胺在体外抑制该酶的活性。亚精胺和精胺也抑制该酶，但效果较差。得出的结论是，该酶与哺乳动物来源的酶相似，同样履行与细胞增殖相关的功能。
相似文献
1
Characterization of ornithine decarboxylase of tobacco cells and tomato ovaries.烟草细胞和番茄子房鸟氨酸脱羧酶的特性研究
Biochem J. 1982 Feb 1;201(2):373-6. doi: 10.1042/bj2010373.
2
The regulation of mouse liver ornithine decarboxylase by metabolites.代谢物对小鼠肝脏鸟氨酸脱羧酶的调节作用。
Biochim Biophys Acta. 1976 Jul 8;438(2):551-62. doi: 10.1016/0005-2744(76)90271-0.
3
Ornithine decarboxylase in Trypanosoma brucei brucei: evidence for selective toxicity of difluoromethylornithine.布氏布氏锥虫中的鸟氨酸脱羧酶：二氟甲基鸟氨酸选择性毒性的证据。
J Protozool. 1982 Aug;29(3):389-94. doi: 10.1111/j.1550-7408.1982.tb05418.x.
4
Ornithine decarboxylase activity in rapidly proliferating plant cells.快速增殖植物细胞中的鸟氨酸脱羧酶活性
FEBS Lett. 1979 Aug 1;104(1):146-8. doi: 10.1016/0014-5793(79)81102-3.
5
Overexpression of arginine decarboxylase in transgenic plants.精氨酸脱羧酶在转基因植物中的过表达。
Biochem J. 1997 Jul 15;325 ( Pt 2)(Pt 2):331-7. doi: 10.1042/bj3250331.
6
Amine-specificity of the inactivating ornithine decarboxylase modification in Physarum polycephalum.多头绒泡菌中失活的鸟氨酸脱羧酶修饰的胺特异性
Biochem J. 1982 Sep 1;205(3):551-7. doi: 10.1042/bj2050551.
7
Diurnal changes in polyamine content, arginine and ornithine decarboxylase, and diamine oxidase in tobacco leaves.烟草叶片中多胺含量、精氨酸和鸟氨酸脱羧酶以及二胺氧化酶的昼夜变化。
J Exp Bot. 2006;57(6):1413-21. doi: 10.1093/jxb/erj121. Epub 2006 Mar 23.
8
Occurrence of ornithine decarboxylase and polyamines in cartilage.软骨中鸟氨酸脱羧酶和多胺的存在情况。
Biochem J. 1977 Feb 15;162(2):347-50. doi: 10.1042/bj1620347.
9
Role of pyridoxal phosphate in mammalian polyamine biosynthesis. Lack of requirement for mammalian S-adenosylmethionine decarboxylase activity.磷酸吡哆醛在哺乳动物多胺生物合成中的作用。对哺乳动物S-腺苷甲硫氨酸脱羧酶活性的需求缺失。
Biochem J. 1977 Jul 15;166(1):81-8. doi: 10.1042/bj1660081.
10
Regulation of ornithine decarboxylase by ODC-antizyme in HTC cells.鸟氨酸脱羧酶抗酶对HTC细胞中鸟氨酸脱羧酶的调控
Biochem Biophys Res Commun. 1977 Apr 25;75(4):948-54. doi: 10.1016/0006-291x(77)91474-7.
引用本文的文献
1
Stereo-Specific Transcript Regulation of the Polyamine Biosynthesis Genes by Enantiomers of Ornithine in Tobacco Cell Culture.烟草细胞培养中鸟氨酸对映体对多胺生物合成基因的立体特异性转录调控
Iran J Biotechnol. 2018 May 15;16(2):e1835. doi: 10.21859/ijb.1835. eCollection 2018 May.
2
Polyamine levels in petunia genotypes with normal and abnormal floral morphologies.矮牵牛正常和异常花形态基因型中的多胺水平。
Plant Physiol. 1988 Feb;86(2):390-3. doi: 10.1104/pp.86.2.390.
3
Polyamine content of long-keeping alcobaca tomato fruit.长保鲜期阿尔卡巴萨番茄果实中的多胺含量。
Plant Physiol. 1988 Feb;86(2):338-40. doi: 10.1104/pp.86.2.338.
4
Assaying ornithine and arginine decarboxylases in some plant species.测定某些植物物种中的鸟氨酸脱羧酶和精氨酸脱羧酶。
Plant Physiol. 1985 Oct;79(2):509-14. doi: 10.1104/pp.79.2.509.
5
Ornithine decarboxylase and arginine decarboxylase activities in meristematic tissues of tomato and potato plants.番茄和马铃薯植株分生组织中鸟氨酸脱羧酶和精氨酸脱羧酶的活性
Plant Physiol. 1982 Aug;70(2):544-6. doi: 10.1104/pp.70.2.544.
6
Participation of ornithine decarboxylase in early stages of tomato fruit development.鸟氨酸脱羧酶参与番茄果实早期发育。
Plant Physiol. 1982 Aug;70(2):540-3. doi: 10.1104/pp.70.2.540.
7
Changes in polyamine biosynthesis associated with postfertilization growth and development in tobacco ovary tissues.烟草子房组织中与受精后生长发育相关的多胺生物合成变化。
Plant Physiol. 1985;79(2):336-43. doi: 10.1104/pp.79.2.336.
8
Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA.一种植物鸟氨酸脱羧酶cDNA的分子克隆与功能鉴定
Biochem J. 1996 Feb 15;314 ( Pt 1)(Pt 1):241-8. doi: 10.1042/bj3140241.
本文引用的文献
1
Polyamine Metabolism in Embryogenic Cells of Daucus carota: I. Changes in Intracellular Content and Rates of Synthesis.胡萝卜胚性细胞中的多胺代谢：I. 细胞内含量和合成速率的变化。
Plant Physiol. 1978 Sep;62(3):430-3. doi: 10.1104/pp.62.3.430.
2
Regulation of nitrate reductase in cultured tobacco cells.培养烟草细胞中硝酸还原酶的调控
Biochim Biophys Acta. 1966 May 5;118(2):299-310. doi: 10.1016/s0926-6593(66)80038-3.
3
Inhibition of pyridoxal enzymes by L-canaline.L-刀豆氨酸对磷酸吡哆醛酶的抑制作用。
Biochim Biophys Acta. 1971 Feb 10;227(2):337-43. doi: 10.1016/0005-2744(71)90065-9.
4
Induction of ornithine decarboxylase in glioma and neuroblastoma cells.胶质瘤和神经母细胞瘤细胞中鸟氨酸脱羧酶的诱导
FEBS Lett. 1976 Sep 15;68(1):63-7. doi: 10.1016/0014-5793(76)80405-x.
5
Ornithine decarboxylase activity in rapidly proliferating plant cells.快速增殖植物细胞中的鸟氨酸脱羧酶活性
FEBS Lett. 1979 Aug 1;104(1):146-8. doi: 10.1016/0014-5793(79)81102-3.
6
The regulation and function of ornithine decarboxylase and of the polyamines.鸟氨酸脱羧酶及多胺的调节与功能
Curr Top Cell Regul. 1979;15:155-202.
7
Production of monospecific antibodies to rat liver ornithine decarboxylase and their use in turnover studies.大鼠肝脏鸟氨酸脱羧酶单特异性抗体的制备及其在周转率研究中的应用。
J Biol Chem. 1977 May 10;252(9):2866-72.
Zotero 插件