Conroy P D, Simms D M, Pointon J J
Biochem J. 1977 Feb 15;162(2):347-50. doi: 10.1042/bj1620347.
The activity of ornithine decarboxylase was investigated in cartilage from chick embryos, rabbits, rats and human foetuses. The enzyme activity in these cartilages was of the same order as the detected in other body tissues. Ornithine decarboxylase activity in chick-embryo cartilage and liver was the same when compared on the basis of total soluble tissue protein. The cartilage enzyme exhibited a pH optimum of 6.5 and a Km for ornithine of 0.16mM. Ornithine decarboxylase activity in chick-embryo pelvic leaflets was maintained at the value in vivo for up to 22h when the isolated tissue was incubated in a modified Waymouth's medium (MB 752/1) at 37 degrees C. After addition of cycloheximide to the incubation medium, ornithine decarboxylase activity declined, with a half-life of 40 min. The concentrations of the polyamines spermidine and spermine in chick-embryo pelvic cartilage and rabbit costal cartilage were of the same order as the concentrations detected in other tissues.
对鸡胚、兔、大鼠和人类胎儿软骨中的鸟氨酸脱羧酶活性进行了研究。这些软骨中的酶活性与在其他身体组织中检测到的活性处于同一水平。以总可溶性组织蛋白为基础进行比较时，鸡胚软骨和肝脏中的鸟氨酸脱羧酶活性相同。软骨酶的最适pH为6.5，鸟氨酸的Km为0.16mM。当分离的鸡胚骨盆叶在改良的威茅斯培养基（MB 752/1）中于37℃孵育时，其鸟氨酸脱羧酶活性在体内水平维持长达22小时。在孵育培养基中加入环己酰亚胺后，鸟氨酸脱羧酶活性下降，半衰期为40分钟。鸡胚骨盆软骨和兔肋软骨中多胺亚精胺和精胺的浓度与在其他组织中检测到的浓度处于同一水平。
