Devdariani T G, Aĭzenberg V L, Bilaĭ T I, Zakordonets L A, Mudzhiri L A
Prikl Biokhim Mikrobiol. 1982 Mar-Apr;18(2):207-11.
The ability of the two cultures Penicillium sp. and Fusarium sp. to synthesize extracellular pectolytic enzymes was investigated. The cultivation conditions providing a high level of the biosynthesis of these enzymes were identified. The methods of isolating the enzymes by means of organic solvents were developed. The pectinase from Penicillium sp. showed a higher thermostability whereas that from Fusarium sp. displayed a greater acid resistance. Using glutaraldehyde and titanium salts, active immobilized forms of pectinases on silica carriers were prepared.
研究了青霉属和镰孢属这两种培养物合成细胞外果胶分解酶的能力。确定了能实现这些酶高水平生物合成的培养条件。开发了利用有机溶剂分离这些酶的方法。青霉属的果胶酶表现出较高的热稳定性，而镰孢属的果胶酶则表现出更强的耐酸性。使用戊二醛和钛盐，制备了硅胶载体上果胶酶的活性固定化形式。
