Faktor V M, Poltoranina V S, Uryvaeva I V
Biull Eksp Biol Med. 1982 Apr;93(4):79-82.
A study was made of cell proliferation and DNA content in the liver of SWR mice after 1, 7, 41 and 52 administrations of CCl4 made at 3-4 daily intervals. After 41 and 52 administrations of CCl4 the liver of all the animals demonstrated multiple small nodules and single large nodes. Cell proliferation in liver and large nodes was analyzed by cytophotometry and radioautography with the use of double isotopic label. The proliferation pattern in the liver was shown to change during cancerogenesis. Unlike normal, a great number of cells were not polyploidized but were reproduced. As regards the cell content, the three adenomatous nodes examined differed from the surrounding parenchyma and from each other. On the whole, the degree of polyploidy in the nodes was much lower despite the fact that the population contained all the cell types, uni- and binuclear cells, up to those with the DNA content of 32c. All these cells and those from the surrounding liver were involved in proliferation. The cellular sources of nodular formations are discussed.
对SWR小鼠肝脏进行了一项研究，该小鼠每隔3 - 4天接受1次、7次、41次和52次四氯化碳注射。在接受41次和52次四氯化碳注射后，所有动物的肝脏均出现多个小结节和单个大结节。使用双同位素标记，通过细胞光度法和放射自显影术分析肝脏和大结节中的细胞增殖情况。结果表明，在致癌过程中肝脏的增殖模式发生了变化。与正常情况不同，大量细胞未多倍体化而是进行了增殖。就细胞含量而言，所检查的三个腺瘤结节与周围实质以及彼此之间均有所不同。总体而言，尽管细胞群体包含所有类型的细胞，单核和双核细胞，直至DNA含量为32c的细胞，但结节中的多倍体程度要低得多。所有这些细胞以及来自周围肝脏的细胞均参与了增殖。文中讨论了结节形成的细胞来源。
