Faktor V M, Uryvaeva I V
Tsitologiia. 1986 Jun;28(6):599-606.
Administration of urethan (three times per 1 mg calculated per 1 g of the animal mass) after partial hepatectomy resulted in the development of liver tumours classified as adenomas in 62.8 per cent of mice 12 months after treatment. In the cells isolated from 85 adenomas and from the surrounding liver, DNA-fuchsin content was determined cytophotometrically. Three types of DNA distribution were distinguished, with the mode in the region of near-diploid (26%), near-tetraploid (71.8%), or near-octaploid (2.2%) DNA value. Most commonly DNA distributions were polymodal, but unimodal ones also occurred. The number of binucleated cells in tumours was significantly decreased. In the liver of one of the same animal, morphologically similar tumours of all the three types of DNA distribution could be found. The increase in the hepatocyte ploidy level in the initial parenchyma by preliminary repeated treatments with CCl4, had no effect either on the occurrence of tumours, or on their ploidy level. No correlation was found between the DNA content, the size and histological structure of tumours.
在部分肝切除术后，按每1克动物体重1毫克的剂量给小鼠注射乌拉坦（分三次注射），治疗12个月后，62.8%的小鼠发生了被归类为腺瘤的肝肿瘤。对从85个腺瘤及其周围肝脏分离出的细胞，用细胞光度法测定了DNA-品红含量。区分出三种类型的DNA分布，其众数处于近二倍体（26%）、近四倍体（71.8%）或近八倍体（2.2%）DNA值区域。DNA分布最常见的是多峰型，但单峰型也有出现。肿瘤中双核细胞的数量显著减少。在同一动物的肝脏中，可以发现具有所有三种DNA分布类型的形态相似的肿瘤。通过用四氯化碳进行初步反复处理使初始实质中的肝细胞倍性水平升高，对肿瘤的发生及其倍性水平均无影响。未发现DNA含量、肿瘤大小和组织结构之间存在相关性。
