Figdor C G, Bont W S, Touw I, de Roos J, Roosnek E E, de Vries J E
Blood. 1982 Jul;60(1):46-53.
Human peripheral blood monocytes were isolated by counterflow centrifugation elutriation (CCE). This technique was modified in such a way that various monocyte fractions (viability greater than 99%) could be elutriated by increasing the density of the CCE-medium in steps of 0.0027 g/ml. All monocytes showed the same size distributions as determined by electronic sizing, which indicated that they differed in their density only. Both cytoplasmic esterase and peroxidase activity increased with the density of the cells. Furthermore, the monocytes with the highest density were 2.3-4 times more active in an antibody-dependent cellular cytotoxicity (ADCC) assay than those with the lowest density. In contrast, the monocyte with the highest density were less capable to induce the proliferation of lymphocytes in mixed leukocyte cultures (MLC) than those with the lowest density. This observation could not be attributed to differences in the expression of HLA-DR determinants, since a monoclonal antibody directed against HLA-DR antigens reacted equally well with the monocytes in different fractions. These results provide evidence for the existence of functionally different subsets of monocytes or different states of differentiation or maturation.
通过逆流离心淘析法（CCE）分离人外周血单核细胞。对该技术进行了改进，通过以0.0027 g/ml的步长增加CCE培养基的密度，能够淘析出不同的单核细胞组分（活力大于99%）。所有单核细胞经电子大小测定显示出相同的大小分布，这表明它们仅在密度上存在差异。细胞质酯酶和过氧化物酶活性均随细胞密度增加而升高。此外，在抗体依赖性细胞毒性（ADCC）试验中，密度最高的单核细胞的活性比密度最低的单核细胞高2.3至4倍。相反，在混合白细胞培养（MLC）中，密度最高的单核细胞诱导淋巴细胞增殖的能力低于密度最低的单核细胞。这一观察结果不能归因于HLA-DR决定簇表达的差异，因为一种针对HLA-DR抗原的单克隆抗体与不同组分中的单核细胞反应同样良好。这些结果为存在功能不同的单核细胞亚群或不同的分化或成熟状态提供了证据。
