Sacks P G, Miller M W
Br J Radiol. 1982 May;55(653):362-5. doi: 10.1259/0007-1285-55-653-362.
Chinese hamster V-79 cells, attached to an acoustically transparent membrane and EMT6/Ro mouse mammary sarcoma cells as small multicellular spheroids or in suspension, were exposed to therapeutic intensities of 1 MHz ultrasound (0-10 W/cm2) and examined for the production of atypical nuclear or chromosomal aberrations. In the monolayer configuration no abnormalities occurred. In multicell spheroids or suspension cells 3 classes of abnormalities were found: (1) pyknotic/irregular nuclei; (2) lysed nuclei with extruding nucleoplasm; and (3) agglomerations which have an irregular shape and "fused" morphology similar to that which has been reported for plant tissues (Cataldo et al, 1973).
附着在透声膜上的中国仓鼠V - 79细胞以及呈小多细胞球体或悬浮状态的EMT6/Ro小鼠乳腺肉瘤细胞，被暴露于1兆赫超声的治疗强度（0 - 10瓦/平方厘米）下，并检测其非典型核或染色体畸变的产生情况。在单层结构中未出现异常。在多细胞球体或悬浮细胞中发现了3类异常情况：（1）固缩/不规则核；（2）有核质挤出的溶解核；（3）具有不规则形状和“融合”形态的团聚物，其形态与已报道的植物组织的形态相似（Cataldo等人，1973年）。
