Barnett S B, Bonin A, Mitchell G, Meher-Homji K M, Baker R S
Br J Radiol. 1982 Jul;55(655):501-4. doi: 10.1259/0007-1285-55-655-501.
The possible mutagenic effect of microsecond pulses of ultrasound was investigated using 3 MHz ultrasound at diagnostic dosages, and also under conditions of increased pulse repetition frequency and acoustic power output. The first part of the study involved 5 strains of Salmonella typhimurium bacteria in which mutagenicity and viability were assessed using the Ames test, while functional competence was evaluated from microscopic observations of motility. No changes were observed in survival, in the incidence of mutants, or in microbial motility following irradiation with ultrasound intensities of 4.5 W/cm2 at temperatures ranging from 37 degrees to 43 degrees C. In the second part of this study, the frequency of sister chromatid exchange (SCE) in Chinese hamster ovary (CHO) cells, treated with similar ultrasound dosages, remained unaltered when cells were irradiated at temperatures up to 41 degrees C. At 43 degrees C and above, cell division was arrested by hyperthermia, an effect unrelated to ultrasound.
使用诊断剂量的3兆赫超声波，并在增加脉冲重复频率和声功率输出的条件下，研究了微秒级超声脉冲可能产生的诱变作用。研究的第一部分涉及5株鼠伤寒沙门氏菌，利用艾姆斯试验评估其诱变性和活力，同时通过显微镜观察运动性来评估功能能力。在37摄氏度至43摄氏度的温度范围内，用4.5瓦/平方厘米的超声强度照射后，未观察到存活率、突变体发生率或微生物运动性的变化。在本研究的第二部分中，当在高达41摄氏度的温度下照射细胞时，用类似超声剂量处理的中国仓鼠卵巢（CHO）细胞中姐妹染色单体交换（SCE）的频率保持不变。在43摄氏度及以上，细胞分裂因热疗而停止，这是一种与超声无关的效应。
