Bing O H, LaRaia P J, Gaasch W H, Spadaro J, Franklin A, Weintraub R M
Circulation. 1982 Aug;66(2 Pt 2):I81-4.
Isolated, blood-perfused dog hearts were subjected to 2 hours of arrest at 27 degrees C and perfused at 15-minute intervals with either a potassium crystalloid cardioplegic solution (KCC) or an identical solution to which washed red blood cells were added (KrbcC). After reperfusion, hearts protected with KCC were easily defibrillated and systolic pressure returned to 62% of prearrest values 90 minutes after reperfusion. In hearts protected with KrbcC, systolic pressures recovered to 103% of prearrest values 15 minutes after reperfusion. Metabolic variables recovered more quickly in hearts perfused with KrbcC. Adenosine triphosphate did not decline with arrest in either group. Creatine phosphate values decreased with KCC and increased with KrbcC. The pH of the myocardial effluent fell to 6.48 during arrest with KCC and to 7.19 with KrbcC (p less than 0.001). Carbon dioxide tension increased with KCC, but decreased with KrbcC (p less than 0.001). Thus, marked improvement in recovery after 2 hours of arrest is seen with KrbcC. Protection beyond that provided by KCC appears solely due to the washed red cells, and may be the result of the buffering action of the red cell improved oxygen delivery.
将离体的、血液灌注的犬心脏在27摄氏度下停搏2小时，然后每隔15分钟用钾晶体心脏停搏液（KCC）或添加了洗涤红细胞的相同溶液（KrbcC）进行灌注。再灌注后，用KCC保护的心脏易于除颤，再灌注90分钟后收缩压恢复到停搏前值的62%。在用KrbcC保护的心脏中，再灌注15分钟后收缩压恢复到停搏前值的103%。用KrbcC灌注的心脏代谢变量恢复得更快。两组中三磷酸腺苷在停搏期间均未下降。磷酸肌酸值在使用KCC时降低，在使用KrbcC时升高。在用KCC停搏期间心肌流出液的pH值降至6.48，用KrbcC时降至7.19（p<0.001）。二氧化碳张力在使用KCC时升高，但在使用KrbcC时降低（p<0.001）。因此，使用KrbcC时，停搏2小时后的恢复情况有显著改善。KrbcC提供的保护作用超出KCC，这似乎完全归因于洗涤红细胞，可能是红细胞缓冲作用以及改善氧输送的结果。
