Tulley R T, Lehmann H P
Clin Chem. 1982 Jul;28(7):1448-50.
In this method of analysis for selenium in whole blood by flameless atomic absorption spectrophotometry, 1 mL of sample is first digested with perchloric and nitric acids. After reduction and neutralization, the sample is reacted with 2,3-diaminonaphthalene, and the product is extracted into toluene. Twenty microliters of the extract is injected into the graphite furnace of a flameless atomic absorption spectrophotometer, along with 20 microliters of a 1.0 g/L cupric nitrate solution. Blood-based standards are used to establish the standard curve. The amount of selenium required to give an absorbance of 0.0044 is 5.3 micrograms/L. Precision is good, recovery excellent. The extract is stable for 24 h.
在这种采用无火焰原子吸收分光光度法分析全血中硒的方法中，首先将1 mL样品用高氯酸和硝酸进行消化。还原并中和后，样品与2,3 - 二氨基萘反应，产物用甲苯萃取。将20微升萃取液与20微升1.0 g/L硝酸铜溶液一起注入无火焰原子吸收分光光度计的石墨炉中。使用血基标准品建立标准曲线。产生吸光度为0.0044所需的硒含量为5.3微克/升。精密度良好，回收率极佳。萃取液在24小时内稳定。
