Oster O, Prellwitz W
Clin Chim Acta. 1982 Sep 30;124(3):277-91. doi: 10.1016/0009-8981(82)90422-3.
Two methods based on different chemical and physical principles for the determination of selenium in serum are elaborated, evaluated and compared--the hydride atomic absorption (AAS) technique and the carbon furnace technique. For the hydride AAS technique a wet destruction method is described. The sensitivity of the method is 10 micrograms Se/1. The carbon furnace technique makes use of the fact that selenium forms refractory selenides when nickel compounds are added. The sensitivity of the carbon furnace technique is 11 micrograms Se/1. The determination of selenium in serum by the hydride AAS and carbon furnace technique correlates satisfactorily (correlation coefficient 0.94; mean value for the hydride AAS 69.1 micrograms Se/1; mean value for the carbon furnace 67.4 micrograms Se/1) indicating that with the carbon furnace technique total selenium (inorganic and organic) is determined. A reference range was established with 99 persons (42 men and 57 women) which show an average selenium content in serum of 80.55 (SD 13.78) micrograms Se/1 [1.02 (0.17) mumol Se/1].
阐述、评估并比较了两种基于不同化学和物理原理测定血清中硒的方法——氢化物原子吸收（AAS）技术和碳炉技术。对于氢化物AAS技术，描述了一种湿式消解方法。该方法的灵敏度为10微克硒/升。碳炉技术利用了添加镍化合物时硒会形成难熔硒化物这一事实。碳炉技术的灵敏度为11微克硒/升。用氢化物AAS和碳炉技术测定血清中的硒具有良好的相关性（相关系数0.94；氢化物AAS的平均值为69.1微克硒/升；碳炉的平均值为67.4微克硒/升），这表明用碳炉技术测定的是总硒（无机硒和有机硒）。对99人（42名男性和57名女性）建立了参考范围，其血清中硒的平均含量为80.55（标准差13.78）微克硒/升[1.02（0.17）微摩尔硒/升]。
