Owens S M, Woodworth J, Mayersohn M
Clin Chem. 1982 Jul;28(7):1509-13.
This accurate, sensitive radioimmunoassay for determining phencyclidine concentrations in serum specimens involves the use of anti-phencyclidine sera, 0.1 mL of serum specimen, an iodinated tracer, and a solid-phase separation. Phencyclidine metabolites do not show significant cross reactivity, but several phencyclidine analogs do cross react. Within-run coefficients of variation for human and dog serum ranged from 2.5 to 13% for concentrations from 2.0 to 500 micrograms/L. Day-to-day coefficients of variation for human and dog serum ranged from 4.3 to 16.7% for concentrations ranging from 2.0 to 90.0 micrograms/L. The sensitivity of the radioimmunoassay is less than 0.5 micrograms/L. Thirty serum specimens from two dogs given 1 mg of phencyclidine per kilogram body weight were analyzed by radioimmunoassay and a gas-chromatographic method. Nonparametric statistical comparison and linear regression showed that results from the two procedures correlate well (r2 = 0.952). Concentration-time data from the two dogs are presented to illustrate the utility of the radioimmunoassay for examining phencyclidine disposition.
这种用于测定血清标本中苯环己哌啶浓度的精确、灵敏的放射免疫分析法，涉及使用抗苯环己哌啶血清、0.1 mL血清标本、一种碘化示踪剂以及固相分离法。苯环己哌啶代谢物没有显著的交叉反应，但几种苯环己哌啶类似物会发生交叉反应。人血清和犬血清的批内变异系数在浓度为2.0至500微克/升时为2.5%至13%。人血清和犬血清的日间变异系数在浓度为2.0至90.0微克/升时为4.3%至16.7%。放射免疫分析法的灵敏度小于0.5微克/升。对两只每千克体重给予1毫克苯环己哌啶的犬的30份血清标本，采用放射免疫分析法和气相色谱法进行了分析。非参数统计比较和线性回归表明，两种方法的结果相关性良好（r2 = 0.952）。给出了两只犬的浓度-时间数据，以说明放射免疫分析法在研究苯环己哌啶处置方面的实用性。
