Osmundsen H, Bremer J
Biochem J. 1978 Aug 15;174(2):379-86. doi: 10.1042/bj1740379.
Rates of acylcarnitine oxidation by isolated heart mitochondria from various animal species were measured polarographically, and by using a spectrophotometric assay [see Osmundsen & Bremer (1977) Biochem. J. 164, 621-633]. Polarographic measurements do not give a correct guide to abilities to beta-oxidize very-long-chain acylcarnitines, in particular C22:1 fatty acylcarnitines. 2. No significant species differences were detected in the abilities to beta-oxidize various C22:1 fatty acylcarnitines. Significant species differences were, however, detected when rates of beta-oxidation were correlated with rates of respiration brought about by very-long-chain acylcarnitines. We concluded that some aspects of oxidative metabolism (possibly the oxidation of tricarboxylic acid-cycle intermediates) are inhibited by very-long-chain fatty acids in some species (e.g. the rat and the cat but not in others (e.g. the pig and the rabbit). 3. It is proposed that the pattern of variation of rates of oxidation of various acylcarnitines (as measured spectrophotometrically) of various chain lengths can be used as a guide to the chain-length specificities of the acyl-CoA dehydrogenases of beta-oxidation (EC 1.3.99.3).
采用极谱法并使用分光光度测定法[见奥斯蒙德森和布雷默（1977年），《生物化学杂志》第164卷，621 - 633页]，测定了来自不同动物物种的离体心脏线粒体对酰基肉碱的氧化速率。极谱测量对于β-氧化超长链酰基肉碱，特别是C22:1脂肪酰基肉碱的能力并不能给出正确的指导。2. 在β-氧化各种C22:1脂肪酰基肉碱的能力方面未检测到显著的物种差异。然而，当β-氧化速率与超长链酰基肉碱引起的呼吸速率相关联时，检测到了显著的物种差异。我们得出结论，在某些物种（如大鼠和猫）中，氧化代谢的某些方面（可能是三羧酸循环中间体的氧化）受到超长链脂肪酸的抑制，而在其他物种（如猪和兔）中则不然。3. 有人提出，通过分光光度法测量的各种不同链长的酰基肉碱氧化速率的变化模式，可以用作β-氧化的酰基辅酶A脱氢酶（EC 1.3.99.3）链长特异性的指导。
