相思豆毒素，来自指腺相思树的毒素。纯化、毒性及体外蛋白质合成抑制作用。
Modeccin, the toxin of Adenia digitata. Purification, toxicity and inhibition of protein synthesis in vitro.
作者信息
Gasperi-Campani A, Barbieri L, Lorenzoni E, Montanaro L, Sperti S, Bonetti E, Stirpe F
出版信息
Biochem J. 1978 Aug 15;174(2):491-6. doi: 10.1042/bj1740491.
Abstract
- Modeccin, the toxin of Adenia digitata (Modecca digitata), was purified from the roots of this plant by affinity chromatography on Sepharose 4B. 2. This toxin is a protein with mol.wt. 57000, which on treatment with 2-mercaptoethanol can be dissociated into two subunits of mol.wts. 25000 and 32000. 3. Modeccin inhibits protein synthesis in vitro in a lysate of rabbit reticulocytes and in Ehrlich ascites cells; the effect on cells is decreased in the presence of lactose. 4. Dissociation of modeccin into subunits decreases the toxicity to animals and the inhibition of protein synthesis in cells, but enhances the inhibition of protein synthesis in the lysate system.
摘要
- 相思豆毒素，即指状腺毛相思子（腺毛相思子）的毒素，通过在琼脂糖4B上进行亲和层析从该植物根部提纯得到。2. 这种毒素是一种分子量为57000的蛋白质，用2-巯基乙醇处理后可解离成分子量分别为25000和32000的两个亚基。3. 相思豆毒素在体外能抑制兔网织红细胞裂解物和艾氏腹水癌细胞中的蛋白质合成；在乳糖存在的情况下，对细胞的作用会减弱。4. 相思豆毒素解离成亚基会降低对动物的毒性以及对细胞中蛋白质合成的抑制作用，但会增强在裂解物系统中对蛋白质合成的抑制作用。
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