Weeda A J, Cohen J
Fertil Steril. 1982 Jun;37(6):817-22. doi: 10.1016/s0015-0282(16)46344-2.
Purification of semen with Sephadex G-50 medium powder or bovine serum albumin (BSA) (20% in medium) columns increases the percentage of motility. Spermatozoa that passed a BSA column or a Sephadex column have significantly higher velocity than unmanipulated spermatozoa. Caffeine (7 mM) stimulation did not show any effect on motility. Neither purification procedures nor stimulation with caffeine improved the in vitro fertilization capacity of capacitated spermatozoa, as determined by interaction with zona-free hamster ova. No difference in in vitro fertilizing capacity could be demonstrated between the first and the last fractions of split ejaculates. After grouping the fractions according to sperm counts, fractions with higher total (motile) sperm counts, and fractions with higher sperm counts had significantly higher fertilization percentages than the fractions with lower (motile) sperm counts.
使用葡聚糖 G-50 介质粉末或牛血清白蛋白（BSA）（培养基中含 20%）柱对精液进行纯化可提高精子活力百分比。通过 BSA 柱或葡聚糖柱的精子速度明显高于未处理的精子。咖啡因（7 mM）刺激对精子活力没有任何影响。通过与去透明带仓鼠卵相互作用测定，纯化程序和咖啡因刺激均未提高获能精子的体外受精能力。在分段射精的第一部分和最后一部分之间，未显示出体外受精能力的差异。根据精子计数对各部分进行分组后，总（活动）精子计数较高的部分以及精子计数较高的部分的受精百分比明显高于（活动）精子计数较低的部分。
