Koch H R, Beitzen R, Kremer F, Chioralia G, Baurmann H, Megaw J, Gardner K, Lerman S
Graefes Arch Clin Exp Ophthalmol. 1982;218(4):193-9. doi: 10.1007/BF02150094.
The effect of systemic 8-methoxypsoralen (8-MOP; 100 mg/kg daily) and subsequent long ultraviolet irradiation (UVA; 300 mJ/cm2; peak: 365 nm) on albino and pigmented rat eyes was studied in a 3-dimensional experimental set-up. While 8-MOP and UVA did not cause any ocular pathology when administered alone, a combined application of the two factors caused reversible corneal opacities, and irreversible iris devascularisation and cataracts. The irreversible changes were seen only in the albinos and accompanied by a significant decrease in lens wet weight. Phosphorescence and EPR spectroscopy demonstrated the formation of an 8-MOP-protein photoadduct in the animals treated with both 8-MOP and UVA. The results of this study emphasize the necessity of shielding the eyes of patients on photochemotherapy with protective spectacles.
在三维实验装置中研究了全身应用8-甲氧基补骨脂素（8-MOP；每日100mg/kg）及随后的长波紫外线照射（UVA；300mJ/cm2；峰值：365nm）对白化病和有色大鼠眼睛的影响。单独给予8-MOP和UVA时未引起任何眼部病变，但联合应用这两种因素会导致可逆性角膜混浊、不可逆性虹膜血管消失和白内障。不可逆性改变仅见于白化病大鼠，且伴有晶状体湿重显著降低。磷光和电子顺磁共振光谱显示，在同时接受8-MOP和UVA治疗的动物中形成了8-MOP-蛋白质光加合物。本研究结果强调了在光化学疗法中用防护眼镜保护患者眼睛的必要性。
