Smith P F, Keefer D A
Horm Metab Res. 1985 Nov;17(11):583-7. doi: 10.1055/s-2007-1013612.
A quantitative autoradiographic technique was combined with immunocytochemical staining to compare 3H-estrogen uptake in individual pituitary cell types 1, 14 or 50 days after castration in both male and female rats. Silver grains were counted over nuclei of immunocytochemically stained cells and means were computed for each cell type. The order of labelling intensity for all groups was gonadotropes greater than or equal to lactotropes = somatotropes greater than thyrotropes = corticotropes. In male rats 3H-estrogen uptake capacity in all of these cell types remained unchanged over the post-castration interval. Only gonadotropes from female rats demonstrated a significant change in estrogen uptake capacity over the intervals examined. Uptake in these cells increased by 137% between 1 and 50 days after ovariectomy. At both 14 and 50 days post-ovariectomy, gonadotropes concentrated significantly more radioactive label than either lactotropes or somatotropes. One day after castration, gonadotropes from females concentrated less 3H-estrogen than males while at 50 days after castration they concentrated significantly more than gonadotropes from male rats.
将定量放射自显影技术与免疫细胞化学染色相结合，以比较雄性和雌性大鼠去势后1天、14天或50天，各垂体细胞类型对³H-雌激素的摄取情况。对免疫细胞化学染色细胞的细胞核进行银颗粒计数，并计算每种细胞类型的平均值。所有组的标记强度顺序为：促性腺激素细胞≥催乳激素细胞 = 生长激素细胞＞促甲状腺激素细胞 = 促肾上腺皮质激素细胞。在雄性大鼠中，所有这些细胞类型的³H-雌激素摄取能力在去势后的时间段内保持不变。在雌性大鼠中，只有促性腺激素细胞在检查的时间段内雌激素摄取能力有显著变化。这些细胞在卵巢切除术后1至50天内摄取量增加了137%。在卵巢切除术后14天和50天，促性腺激素细胞积累的放射性标记明显多于催乳激素细胞或生长激素细胞。去势后1天，雌性促性腺激素细胞积累的³H-雌激素比雄性少，而去势后50天，它们积累的³H-雌激素明显多于雄性大鼠的促性腺激素细胞。
