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基质载体上的角膜上皮细胞培养物。

Corneal epithelial cell cultures on stromal carriers.

作者信息

Friend J, Kinoshita S, Thoft R A, Eliason J A

出版信息

Invest Ophthalmol Vis Sci. 1982 Jul;23(1):41-9.

PMID:7085220
Abstract

Exposure of denuded rabbit corneal stromal carriers for 24 hr at 37 degrees C to suspensions of rabbit corneal epithelial cells grown in tissue culture resulted in the establishment of a cell layer on the carriers. The cell layers persisted for at least 1 week of incubation and were one to three cells thick. They consisted of healthy-appearing cells with normal intracellular organelles and intercellular desmosomal connections. After 2 to 7 days of incubation the cells were still capable of DNA replication and produced hemidesmosomes and basement membrane. This system is useful for in vitro studies of substrate requirements for hemidesmosome and basement-membrane formation by corneal epithelial cells.

摘要

将兔角膜基质载体在37摄氏度下暴露于组织培养中生长的兔角膜上皮细胞悬液24小时,导致载体上形成细胞层。细胞层在培养至少1周后仍持续存在,厚度为1至3个细胞。它们由外观健康的细胞组成,具有正常的细胞内细胞器和细胞间桥粒连接。培养2至7天后,细胞仍能够进行DNA复制,并产生半桥粒和基底膜。该系统可用于角膜上皮细胞形成半桥粒和基底膜的底物需求的体外研究。

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