Bu'Lock A J, Vaillant C, Dockray G J, Bu'Lock J D
Histochemistry. 1982;74(1):49-55. doi: 10.1007/BF00495051.
Study of the possible chemical mechanisms by which parabenzoquinone acts as a fixative in the localisation of neuronal peptides by immunohistochemistry suggested several possible improvements to standard techniques. In particular, by using favourable conditions for regenerative oxidation reactions, i.e. fixing at 37 degrees C in well oxygenated, slightly alkaline buffer, it was possible to improve the localisation of substance P-, vasoactive intestinal polypeptide-, enkephalin- and somatostatin-like immunoreactive peptides such that nerve cell bodies were routinely visualised without the use of drugs to block axonal transport. Application of the improved fixation method is likely to facilitate study of the organisation of peptidergic systems.
对羟基苯醌在免疫组织化学中作为神经元肽定位固定剂的可能化学机制的研究表明，标准技术有几个可能的改进之处。特别是，通过利用有利于再生氧化反应的条件，即在37摄氏度、充分充氧的微碱性缓冲液中固定，可以改善P物质、血管活性肠多肽、脑啡肽和生长抑素样免疫反应性肽的定位，从而在不使用药物阻断轴突运输的情况下常规观察到神经细胞体。应用改进的固定方法可能有助于肽能系统组织的研究。
