Lyon E S, Jakoby W B
J Biol Chem. 1982 Jul 10;257(13):7531-5.
Arylamine N-methyltransferase catalyzes the novel methylation of the ring nitrogen of tryptamine and pyrrole as well as a number of other arylamines including aniline and its derivatives. S-Adenosyl-L-methionine serves as donor. Tyramine, indole, benzylamine, and desmethylimipramine were inactive as methyl group acceptors. The enzyme was purified from rabbit liver to electrophoretic homogeneity and was characterized as a protein monomer of 27,000 daltons with a pI of 4.8. The kinetic mechanism appears to be rapid equilibrium random Bi-Bi.
芳胺N-甲基转移酶催化色胺和吡咯的环氮以及包括苯胺及其衍生物在内的许多其他芳胺的新型甲基化反应。S-腺苷-L-甲硫氨酸作为供体。酪胺、吲哚、苄胺和去甲丙咪嗪作为甲基受体无活性。该酶从兔肝脏中纯化至电泳纯，其特征为27,000道尔顿的蛋白质单体，pI为4.8。动力学机制似乎是快速平衡随机双底物双产物机制。
