高效液相色谱法测定生物体液中的嘌呤和嘧啶碱基、核糖核苷、脱氧核糖核苷及环核苷酸。
High-performance liquid chromatographic determination of purine and pyrimidine bases, ribonucleosides, deoxyribonucleosides and cyclic ribonucleotides in biological fluids.
作者信息
De Abreu R A, Van Baal J M, De Bruyn C H, Bakkeren J A, Schretlen E D
出版信息
J Chromatogr. 1982 Apr 16;229(1):67-75. doi: 10.1016/s0378-4347(00)86037-5.
A method is presented for the separation and quantitative determination of compounds normally related to purine and pyrimidine metabolism in biological material. The retention behaviour of nucleobases, ribonucleosides, deoxyribonucleosides and cyclic ribonucleotides has been systematically investigated by reversed-phase high-performance liquid chromatography using a non-linear gradient. Ultimately a separation of the purine and pyrimidine compounds was achieved in a 35-min run with an average detection limit of 5-10 pmol per injection. Recoveries of standards added to urine, plasma or serum were 96 +/- 5%.
本文介绍了一种用于分离和定量测定生物材料中通常与嘌呤和嘧啶代谢相关的化合物的方法。通过使用非线性梯度的反相高效液相色谱法，系统地研究了核碱基、核糖核苷、脱氧核糖核苷和环核糖核苷酸的保留行为。最终，在35分钟的运行中实现了嘌呤和嘧啶化合物的分离，每次进样的平均检测限为5-10皮摩尔。添加到尿液、血浆或血清中的标准品回收率为96±5%。
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