Eells J T, Spector R
Neurochem Res. 1983 Oct;8(10):1307-20. doi: 10.1007/BF00964000.
Purine and pyrimidine base and nucleoside levels were measured in adult rabbit cisternal CSF and plasma by reversed-phase high-performance liquid chromatography. The concentrations of bases, nucleosides, and nucleoside phosphates were similar in plasma and CSF except for the adenosine phosphates and uracil which were higher in the plasma. In plasma and CSF, adenosine levels were low (0.12 microM) and guanosine, deoxyadenosine, deoxyguanosine, and deoxyinosine were not detectable (less than 0.1 microM); inosine and xanthine concentrations were 1-2 microM and hypoxanthine concentrations were approximately 5 microM; uridine (approximately 8 microM), cytidine (2-3 microM), and thymidine, deoxyuridine, and deoxycytidine (0.5-1.4 microM) were easily detectable. In both plasma and CSF, guanine, and thymine were undetectable (less than 0.1 microM), adenine and cytosine were less than 0.2 microM, but uracil was present (greater than 1 microM). Adenosine, inosine, and guanosine phosphates were also detectable at low concentrations in CSF and plasma. These results are consistent with the hypothesis that purine deoxyribonucleosides are synthesized in situ in the adult rabbit brain. In contrast, pyrimidine deoxyribonucleosides and ribonucleosides, and purine and pyrimidine bases are available in the CSF for use by the brain.
采用反相高效液相色谱法测定成年兔脑池脑脊液和血浆中的嘌呤和嘧啶碱基及核苷水平。除血浆中磷酸腺苷和尿嘧啶含量较高外,血浆和脑脊液中碱基、核苷及核苷磷酸的浓度相似。在血浆和脑脊液中,腺苷水平较低(0.12微摩尔),鸟苷、脱氧腺苷、脱氧鸟苷和脱氧肌苷无法检测到(低于0.1微摩尔);肌苷和黄嘌呤浓度为1 - 2微摩尔,次黄嘌呤浓度约为5微摩尔;尿苷(约8微摩尔)、胞苷(2 - 3微摩尔)以及胸苷、脱氧尿苷和脱氧胞苷(0.5 - 1.4微摩尔)易于检测到。在血浆和脑脊液中,鸟嘌呤和胸腺嘧啶无法检测到(低于0.1微摩尔),腺嘌呤和胞嘧啶低于0.2微摩尔,但尿嘧啶存在(大于1微摩尔)。脑脊液和血浆中也能检测到低浓度的腺苷、肌苷和鸟苷磷酸。这些结果与嘌呤脱氧核糖核苷在成年兔脑中原位合成的假说一致。相比之下,嘧啶脱氧核糖核苷和核糖核苷以及嘌呤和嘧啶碱基可在脑脊液中供大脑使用。