Summerly R, Ilderton E, Gray G M
Br J Dermatol. 1978 Sep;99(3):279-88. doi: 10.1111/j.1365-2133.1978.tb01997.x.
Biopsies of normal skin, psoriatic lesions and the adjacent psoriatic skin were incubated with I-14C-acetate, 32P-orthophosphate and U-14C-glycerol. Total incorporation of I-14C-acetate into psoriatic lesions (17 samples) was 50% higher than in the adjacent uninvolved epidermis (9 samples) and 120% higher than into normal epidermis (10 samples). In the psoriatic lesion a much higher proportion of the total incorporation was into the neutral lipids and was due mainly to a very high incorporation of I-14C-acetate into the triacylglycerols. The I-14C-acetate incorporated into the phospholipids and especially phosphatidylcholine was proportionally much less in the psoriatic lesion and uninvolved psoriatic epidermis than in normal epidermis even though the incorporation of 32P-orthophosphate and U-14C-glycerol, both representing de novo synthesis, into the phospholipids in the psoriatic lesions and uninvolved epidermis were higher (four-fold in lesion) than in normal epidermis. Our findings (1) are evidence for a much increased triacylglycerol synthesis in psoriatic epidermis which would account for the long-known observation of lipid droplet accumulation in psoriatic cells; (2) suggest that in psoriatic epidermis there is a defect in phospholipid metabolism mainly involving phosphatidycholine and the deacylation (phospholipase A)-reacylation (phospholipid acyltransferase) cycle for fatty acid transfer.
将取自正常皮肤、银屑病皮损及相邻银屑病皮肤的活检组织与I-14C-乙酸盐、32P-正磷酸盐和U-14C-甘油一起孵育。I-14C-乙酸盐在银屑病皮损（17个样本）中的总掺入量比相邻未受累表皮（9个样本）高50%，比正常表皮（10个样本）高120%。在银屑病皮损中，总掺入量中有更高比例进入中性脂质，这主要是由于I-14C-乙酸盐大量掺入三酰甘油。尽管代表从头合成的32P-正磷酸盐和U-14C-甘油在银屑病皮损和未受累表皮中的磷脂掺入量高于正常表皮（皮损中高四倍），但在银屑病皮损和未受累银屑病表皮中，掺入磷脂尤其是磷脂酰胆碱的I-14C-乙酸盐比例比正常表皮少得多。我们的研究结果：（1）证明银屑病表皮中三酰甘油合成大幅增加，这可以解释长期以来已知的银屑病细胞中脂滴积累现象；（2）表明银屑病表皮中磷脂代谢存在缺陷，主要涉及磷脂酰胆碱以及脂肪酸转移的去酰化（磷脂酶A）-再酰化（磷脂酰基转移酶）循环。
