Kubín M, Sedlácková J, Vacek K
J Hyg Epidemiol Microbiol Immunol. 1982;26(1):31-6.
Sterile drinking water samples were artificially colonized with M. Kansasii, M. gordonae and M. fortuitum suspensions (the numbers of viable unite in 1 ml were 1.2 x 10(3), 48.5 and 3.2 x 10(3), respectively) prepared from mycobacterial strains replicated in Tween 80-free liquid Dubos medium STO. The contaminated water samples were irradiated from a rotary cobalt-60 source (gamma radiation, E = 1.17 and 1.33 MeV, dose intensity 1 kJ/kg.h at room temperature) with doses 0.7, 1.5, 2.2, 3, 9, 16 and 27 kJ/kg. The disinfecting effectiveness was assessed by direct cultivation tests (0.5 ml volumes of water inoculated on egg medium) and by cultivation on membrane filters after filtrating the whole amount of the water examined (about 500 ml). Fully positive disinfecting effects were recorded in M. kansasii and M fortuitum irradiated with 9 kJ/kg and in M. gordonae after irradiation with 1.5 kJ/kg. The calculated value of D10 = 0.4 kJ/kg (i.e. the dose radiation that reduces the number if viable mycobacteria by an order of magnitude) is suggestive of a strong disinfecting effect of ionizing radiation on the tested strains of potentially pathogenic mycobacteria. The results indicate that the use of ionizing radiation to disinfect mycobacteria that are difficult to remove with other methods which, as a rule, cannot ensure the permanent disinfection.
无菌饮用水样本用堪萨斯分枝杆菌、戈登分枝杆菌和偶然分枝杆菌悬液进行人工接种（每毫升活菌数分别为1.2×10³、48.5和3.2×10³），这些悬液由在不含吐温80的液体杜博斯培养基STO中传代培养的分枝杆菌菌株制备而成。受污染的水样用旋转钴-60源进行辐照（γ辐射，E = 1.17和1.33 MeV，室温下剂量强度为1 kJ/kg·h），剂量分别为0.7、1.5、2.2、3、9、16和27 kJ/kg。通过直接培养试验（将0.5毫升水样接种到鸡蛋培养基上）以及对所检测的全部水样（约500毫升）过滤后在膜滤器上进行培养来评估消毒效果。在用9 kJ/kg辐照的堪萨斯分枝杆菌和偶然分枝杆菌以及用1.5 kJ/kg辐照的戈登分枝杆菌中记录到了完全阳性的消毒效果。计算得出的D10值为0.4 kJ/kg（即能使活菌数减少一个数量级的辐射剂量），这表明电离辐射对所测试的潜在致病性分枝杆菌菌株具有很强的消毒作用。结果表明，使用电离辐射对用其他方法难以去除的分枝杆菌进行消毒，而其他方法通常无法确保永久性消毒。
