Clausen O P, Thorud E, Iversen O H
J Invest Dermatol. 1982 Jun;78(6):472-6. doi: 10.1111/1523-1747.ep12510172.
The cell kinetic response after intraperitoneal injection of the 10 micrograms adrenalin was investigated in hairless mouse epidermis. Changes in the proportion of cells in S and G2 phase were studied by means of flow cytometry of isolated basal cells. Changes in the proportion of cells in prophase and metaphase, changes in the mitotic rate (Colcemid method) and in cell cycle progression of 3H-TdR labeled cells were studied in histologic sections. The results showed that adrenalin has a differential effect on cell proliferation in mouse epidermis. The cell progression rate from S phase through G2 phase to metaphase is increased in one cohort of cells shortly after adrenalin injection. Simultaneously another cohort of cells is reversibly delayed or blocked in prophase. In agreement with most previous studies a significantly reduced cell division rate was seen 2-3 hr after adrenalin injection. At this time the proportions of cells in prophase and G2 phase were normalized, whereas a significant increase in the proportion of cells in S phase had cycle progression out of S phase might be responsible for the reduced mitotic rate seen after adrenalin administration.
在无毛小鼠表皮中研究了腹腔注射10微克肾上腺素后的细胞动力学反应。通过对分离的基底细胞进行流式细胞术研究S期和G2期细胞比例的变化。在组织学切片中研究前期和中期细胞比例的变化、有丝分裂率（秋水仙酰胺法）的变化以及3H-胸腺嘧啶核苷标记细胞的细胞周期进程。结果表明，肾上腺素对小鼠表皮细胞增殖具有差异效应。在注射肾上腺素后不久，一组细胞从S期经G2期到中期的细胞进程速率增加。同时，另一组细胞在前期间可逆地延迟或阻滞。与大多数先前研究一致，在注射肾上腺素后2 - 3小时观察到细胞分裂率显著降低。此时，前期和G2期细胞的比例恢复正常，而S期细胞的比例显著增加，S期外的细胞周期进程可能是肾上腺素给药后有丝分裂率降低的原因。
