Halonen T, Näntö V, Frey H, Lövgren T
J Neurochem. 1982 Jul;39(1):36-43. doi: 10.1111/j.1471-4159.1982.tb04698.x.
A bioluminescent assay based on the firefly luciferase reaction has been used for determination of creatine kinase activity in CSF. Activities as low as 0.1 U/L can be measured. The coefficient of variation at an activity level of 0.3-0.4 U/L was between 5 and 6%. The assay conditions optimized for serum specimens can be used for CSF. The adenylate kinase activity is almost completely inhibited, which simplifies the procedure. The creatine kinase/(CK) isoenzyme distribution was obtained using the bioluminescent assay in combination with immunoinhibition or ion exchange chromatography. All specimens contained both MM and BB activity, but no MB was found. The study indicates that the bioluminescent assay is useful in the determination of CK isoenzymes in CSF. The clinical importance of the observed CK levels will be reported in a separate communication.
一种基于萤火虫荧光素酶反应的生物发光测定法已用于测定脑脊液中的肌酸激酶活性。可测量低至0.1 U/L的活性。在0.3 - 0.4 U/L的活性水平下，变异系数在5%至6%之间。针对血清标本优化的测定条件可用于脑脊液。腺苷酸激酶活性几乎被完全抑制，这简化了操作过程。使用生物发光测定法结合免疫抑制或离子交换色谱法获得了肌酸激酶（CK）同工酶分布。所有标本都含有MM和BB活性，但未发现MB。该研究表明，生物发光测定法在脑脊液中CK同工酶的测定中很有用。观察到的CK水平的临床重要性将在另一篇通讯中报道。
