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培养细胞的多重延时显微摄影。

Multiplexed time-lapse photomicrography of cultured cells.

作者信息

Heye R R, Kiebler E W, Arnzen R J, Tolmach L J

出版信息

J Microsc. 1982 Jan;125(Pt 1):41-50. doi: 10.1111/j.1365-2818.1982.tb00321.x.

DOI:10.1111/j.1365-2818.1982.tb00321.x
PMID:7086877
Abstract

A system of cinemicrography has been developed in which a single microscope and 16 mm camera are multiplexed to produce a time-lapse photographic record of many fields simultaneously. The field coordinates and focus are selected via a control console and entered into the memory of a dedicated microcomputer; they are then automatically recalled in sequence, thus permitting the photographing of additional fields in the interval between exposures of any given field. Sequential exposures of each field are isolated in separate sections of the film by means of a specially designed random-access camera that is also controlled by the microcomputer. The need to unscramble frames is thereby avoided, and the developed film can be directly analysed.

摘要

已经开发出一种显微电影摄影系统,其中一台显微镜和一台16毫米摄像机多路复用,以同时产生许多视野的延时摄影记录。通过控制台选择视野坐标和焦点,并输入到专用微型计算机的存储器中;然后按顺序自动调出这些坐标和焦点,从而允许在任何给定视野的曝光间隔内拍摄其他视野。借助同样由微型计算机控制的专门设计的随机存取相机,每个视野的连续曝光被隔离在胶片的不同部分。从而避免了对帧进行解扰的需要,并且可以直接分析冲洗后的胶片。

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