Kallman R F, Blevins N, Coyne M A, Prionas S D
Department of Radiation Oncology, Stanford University Medical School, California 94305.
Comput Biomed Res. 1990 Apr;23(2):115-29. doi: 10.1016/0010-4809(90)90011-z.
The most significant feature of the system that is described is its ability to image essentially simultaneously the growth of up to 99 single cells into macroscopic colonies, each in its own microscope field. Operationally, fields are first defined and programmed by a trained observer. All subsequent steps are automatic and under computer control. Salient features of the hardware are stepper motor-controlled movement of the stage and fine adjustment of an inverted microscope, a high-quality 16-mm cine camera with light meter and controls, and a miniature incubator in which cells may be grown under defined conditions directly on the microscope stage. This system, termed MUTLAS, necessitates reordering of the primary images by rephotographing them on fresh film. Software developed for the analysis of cell and colony growth requires frame-by-frame examination of the secondary film and the use of a mouse-driven cursor to trace microscopically visible (4X objective magnification) events.
所描述系统的最重要特征是其能够基本同时对多达99个单细胞生长为宏观菌落的过程进行成像，每个细胞在其自己的显微镜视野中。在操作上，视野首先由经过训练的观察者定义和编程。所有后续步骤都是自动的且由计算机控制。硬件的显著特征包括：由步进电机控制的载物台移动和倒置显微镜的精细调节、带有光度计和控制装置的高质量16毫米电影摄像机，以及一个微型培养箱，细胞可在特定条件下直接在显微镜载物台上生长。这个称为MUTLAS的系统需要通过在新胶片上重新拍摄来对原始图像进行重新排序。为分析细胞和菌落生长而开发的软件需要逐帧检查二次胶片，并使用鼠标驱动的光标来追踪显微镜下可见（4倍物镜放大倍数）的事件。
