噬菌体T4转导噬菌体的遗传分析。
Genetic analysis of bacteriophage T4 transducing bacteriophages.
作者信息
Young K K, Edlin G J, Wilson G G
出版信息
J Virol. 1982 Jan;41(1):345-7. doi: 10.1128/JVI.41.1.345-347.1982.
Abstract
Mutations in the genes for nuclear disruption (ndd), endonuclease IV (denB), and the D1 region of the T4 genome are essential for converting bacteriophage T4 into a generalized transducing phage. These mutations gave rise to a very low frequency of transduction, about 10(-8) per infected bacterium. The addition of an rII mutation raised the transduction frequency about 20-fold. An additional 100-fold increase in the transduction frequency was observed with mutations in genes 42, 56, and alc. High-frequency generalized transduction by T4 results from the cumulative effect of these mutations.
摘要
核破坏基因（ndd）、核酸内切酶IV（denB）以及T4基因组D1区域的基因突变对于将噬菌体T4转化为广义转导噬菌体至关重要。这些突变导致转导频率非常低，约为每个受感染细菌10^(-8) 。添加rII突变可使转导频率提高约20倍。在基因42、56和alc发生突变时，转导频率又额外增加了100倍。T4的高频广义转导是这些突变累积效应的结果。
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