Snow J W, Brandts J F, Low P S
Biochim Biophys Acta. 1978 Oct 4;512(3):579-91. doi: 10.1016/0005-2736(78)90167-0.
Red blood cell membranes have been labeled with several covalent and noncovalent inhibitors of anion transport and their heat capacity profiles determined as a function of temperature. Covalent inhibitors include the amino reactive agents 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, pyridoxal phosphate and 1-fluoro-2,4-dinitro benzene. The non-covalent inhibitors include several well known local anesthetics. The study was underataken in order to identify regions of the membrane involved in anion transport. Covalent modification in all case resulted in a large upward shift of the C transition, which is beleived to involve a localized phospholipid region. Evidence is presented which indicates that Band III protein and this phospholipid region are in close physical proximity on the membrane. Addition of non-covalent inhibitors affects the membrane in either or both of two ways. In some cases, a lowering and broadening of the C transition occurs; in others the B1 and B2 transitions are altered. These latter transitions are beleived to involve both phospholipid and protein, including Band III. These results may indicate that the non-covalent inhibitors produce their inhibitory effect on anion transport at least in part by interacting with membrane phospholipid.
红细胞膜已用几种阴离子转运的共价和非共价抑制剂进行标记，并测定了它们的热容量随温度变化的曲线。共价抑制剂包括氨基反应剂4,4'-二异硫氰基芪-2,2'-二磺酸、4-乙酰氨基-4'-异硫氰基芪-2,2'-二磺酸、磷酸吡哆醛和1-氟-2,4-二硝基苯。非共价抑制剂包括几种著名的局部麻醉剂。进行这项研究是为了确定膜中参与阴离子转运的区域。在所有情况下，共价修饰都会导致C转变大幅向上移动，据信这涉及一个局部磷脂区域。有证据表明，带III蛋白和这个磷脂区域在膜上紧密相邻。添加非共价抑制剂以两种方式中的一种或两种影响膜。在某些情况下，会发生C转变的降低和展宽；在其他情况下，B1和B2转变会改变。后一种转变据信涉及磷脂和蛋白质，包括带III。这些结果可能表明，非共价抑制剂至少部分地通过与膜磷脂相互作用对阴离子转运产生抑制作用。
