Lang R, Adler I D
Mutat Res. 1982 Feb 22;92(1-2):243-8. doi: 10.1016/0027-5107(82)90227-5.
Chlordimeforme and its 2 principal metabolites, N-formyl-4-chloro-o-toluidine and 4-chloro-o-toluidine, were studied for induction of heritable translocations. Maximal tolerated doses of the compounds were given daily by gavage for 7 consecutive weeks. After mating with untreated females, about 1000 F1 male offspring per group--including vehicle control and positive control (Tretamine, TEM)--were tested for their reproductive performance by use of a sequential decision procedure on litter sizes to select males with translocation heterozygosity. Partially sterile, sterile and non-classifiable F1 males were examined cytogenetically by scoring meiotic chromosomes for translocation multivalents or analysing mitotic divisions for marker chromosomes. The 3 compounds tested at dose levels showing toxic effects did not induce translocation heterozygosity. Tretamine, the positive control, gave the expected mutagenic response.
研究了杀虫脒及其两种主要代谢物N-甲酰-4-氯邻甲苯胺和4-氯邻甲苯胺对可遗传易位的诱导作用。连续7周每天经口灌胃给予化合物的最大耐受剂量。与未处理的雌性交配后，每组约1000只F1雄性后代——包括溶剂对照和阳性对照（三亚乙基三聚氰胺，TEM）——通过对窝仔数采用序贯决策程序来测试其生殖性能，以选择具有易位杂合性的雄性。对部分不育、不育和不可分类的F1雄性进行细胞遗传学检查，通过对减数分裂染色体上的易位多价体进行评分或对有丝分裂进行分析以检测标记染色体。在显示出毒性作用的剂量水平下测试的这3种化合物未诱导易位杂合性。阳性对照三亚乙基三聚氰胺产生了预期的诱变反应。
